Title:
Bone marrow regeneration follwing tibial marrow ablation in rats is age dependent

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dc.contributor.advisor Boyan, Barbara D.
dc.contributor.author Fisher, Maya en_US
dc.contributor.committeeMember Guldberg Robert
dc.contributor.committeeMember Lobachev, Kirill
dc.contributor.committeeMember Schwartz Zvi
dc.contributor.department Biology en_US
dc.date.accessioned 2009-01-22T15:43:03Z
dc.date.available 2009-01-22T15:43:03Z
dc.date.issued 2008-11-19 en_US
dc.description.abstract Objective: Injuries to the marrow cavity result in rapid bone formation followed by regeneration of the marrow. It is not known whether this process is affected by age, although the quality of marrow is markedly different in young and old animals. To test if marrow restoration differs with age, we used the rat tibial bone marrow ablation model, which has been used to examine calcification, osteointegration of metal implants, and remodeling of bone graft substitutes. Methods: Marrow was ablated in the left tibia of seven rats (rNu/rNu) per time point. At 0,7,14,21,28,35 and 42 days post-surgery, treated tibias and contralateral tibias were harvested and fixed in buffered formalin. Both tibias were scanned using microCT and trabecular and cortical BVF/TV calculated. Mid-sagittal sections of decalcified bones were stained with H&E and BVF/TV calculated. Results: MicroCT analysis of 1-month animals showed increased bone formation on day-7 and on day-21 the marrow was restored. Increased bone was seen in 3-month animals on day-7 and day-14, but it was significantly less than in 1-month rats. By day-21, trabecular bone was reduced by 50%. 10-month animals had less trabecular bone at day-7 and 14, but bone remained in the medullary canal through day-1. Histomorphometry indicated that bone formation peaked at day-7 in 1-month rats with remodeling underway by day-14. Bone formation in 3-month rats also peaked at day-7, but restoration occurred by day-21. However, in 10-month rats, peak bone occurred on day-14, with remodeling on day-28. Conclusions: Aged animals produced less primary bone than younger animals and remodeling was initiated later. Differences in micro-CT and histomorphometric analyses may reflect a reduction in calcification of the osteoid in the 10-month old animals. (Supported by Boston Scientific, Inc.) en_US
dc.description.degree M.S. en_US
dc.identifier.uri http://hdl.handle.net/1853/26526
dc.publisher Georgia Institute of Technology en_US
dc.subject Ablation model en_US
dc.subject Trabecular bone en_US
dc.subject Bone marrow en_US
dc.subject Aging en_US
dc.subject.lcsh Bone regeneration
dc.subject.lcsh Regeneration (Biology)
dc.subject.lcsh Developmental biology
dc.title Bone marrow regeneration follwing tibial marrow ablation in rats is age dependent en_US
dc.type Text
dc.type.genre Thesis
dspace.entity.type Publication
local.contributor.corporatename College of Sciences
local.contributor.corporatename School of Biological Sciences
relation.isOrgUnitOfPublication 85042be6-2d68-4e07-b384-e1f908fae48a
relation.isOrgUnitOfPublication c8b3bd08-9989-40d3-afe3-e0ad8d5c72b5
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