Characterizing iron reducing microorganisms from anoxic ferruginous lake sediments

dc.contributor.advisor Glass, Jennifer B.
dc.contributor.author Costa, Bianca Francesca
dc.contributor.committeeMember Pavlostathis, Spyros G.
dc.contributor.department Earth and Atmospheric Sciences
dc.date.accessioned 2019-02-12T14:43:01Z
dc.date.available 2019-02-12T14:43:01Z
dc.date.created 2018-12
dc.date.issued 2018-12
dc.date.submitted December 2018
dc.date.updated 2019-02-12T14:43:01Z
dc.description.abstract Lake Matano, Indonesia is a well-known ancient ocean analogue as its anoxic ecosystem in the subsurface sediments allow the growth of microorganisms capable of mediating anaerobic oxidation coupled to iron reduction. An anoxic, ferric iron rich BSR was kept for 163 days to culture iron reducing bacteria in an environmental sample from Lake Matano sediment. Microscopy analysis was performed periodically to look for morphology trends using DAPI staining. Phylogenetic analysis was performed using CARD-FISH microscopy to target the Geobacter genus, known for electroactive abilities through pilA and interactions with iron particles. Cell counts correlated with iron reduction throughout the culturing period indicating iron reducing microorganisms were present in the environmental sample. Filamentous, coccus, bacillus and vibrio morphologies were observed through the culturing period with vibrio and bacillus being predominant at later stages of the culture. Particle association instances were observed at days 20, 62, 86 and 126 and DIET interactions at day 86. Characterizing these microorganisms opens a broad range of possibilities for wastewater treatment (strip mining groundwater contamination), use in bioelectrochemical systems biofilms (due to external electron transport pilA) and understanding the importance of this microorganisms in Iron cycling.
dc.description.degree Undergraduate
dc.format.mimetype application/pdf
dc.identifier.uri http://hdl.handle.net/1853/60891
dc.language.iso en_US
dc.publisher Georgia Institute of Technology
dc.subject CARD-FISH
dc.subject Iron Reducing bacteria
dc.subject characterization
dc.title Characterizing iron reducing microorganisms from anoxic ferruginous lake sediments
dc.type Text
dc.type.genre Undergraduate Thesis
dspace.entity.type Publication
local.contributor.advisor Glass, Jennifer B.
local.contributor.corporatename School of Earth and Atmospheric Sciences
local.contributor.corporatename College of Sciences
local.relation.ispartofseries Undergraduate Research Option Theses
relation.isAdvisorOfPublication 46762027-c870-495e-a053-b7b8ffdc0730
relation.isOrgUnitOfPublication b3e45057-a6e8-4c24-aaaa-fb00c911603e
relation.isOrgUnitOfPublication 85042be6-2d68-4e07-b384-e1f908fae48a
relation.isSeriesOfPublication e1a827bd-cf25-4b83-ba24-70848b7036ac
thesis.degree.level Undergraduate
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