Ribonucleotides embedded in yeast genomic DNA are targets of RNase H2 and the nucleotide excision repair system

dc.contributor.advisor Storici, Francesca
dc.contributor.author Gombolay, Alli
dc.contributor.committeeMember Lobachev, Kirill
dc.contributor.committeeMember Montoya, Joseph
dc.contributor.department Biology
dc.date.accessioned 2014-10-31T12:26:57Z
dc.date.available 2014-10-31T12:26:57Z
dc.date.issued 2013-04
dc.description.abstract Increasing evidence suggests that ribonucleotides may represent one of the most common non-standard nucleotides found in genomic DNA. Therefore, it is important to understand the extent to which ribonucleotides alter genomic integrity and the cellular mechanisms that are responsible for removing them. We developed oligonucleotide-driven gene correction assays in the yeast Saccharomyces cerevisiae to show that, if not removed, mispaired and paired ribonucleotides embedded in genomic DNA serve as templates for DNA synthesis and could cause genetic change. We found that RNase H type 2 targets single paired and mispaired ribonucleotides, as well as a stretch of two or three ribonucleotides embedded in DNA, and the nucleotide excision repair system can target single paired ribonucleotides as damage. en_US
dc.embargo.terms null en_US
dc.identifier.uri http://hdl.handle.net/1853/52785
dc.language.iso en_US en_US
dc.publisher Georgia Institute of Technology en_US
dc.subject Double strand break repair en_US
dc.subject NER en_US
dc.subject DSB en_US
dc.title Ribonucleotides embedded in yeast genomic DNA are targets of RNase H2 and the nucleotide excision repair system en_US
dc.type Text
dc.type.genre Undergraduate Thesis
dspace.entity.type Publication
local.contributor.advisor Storici, Francesca
local.contributor.corporatename College of Sciences
local.contributor.corporatename School of Biological Sciences
local.contributor.corporatename Undergraduate Research Opportunities Program
local.relation.ispartofseries Undergraduate Research Option Theses
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