Cell Contraction Induces Long-Ranged Stress Stiffening in the Extracellular Matrix

Ronceray, Pierre; Han, Yu Long; Lenz, Martin; Broedersz, Chase; Guo, Ming

Title:

Cell Contraction Induces Long-Ranged Stress Stiffening in the Extracellular Matrix

dc.contributor.author	Ronceray, Pierre
dc.contributor.author	Han, Yu Long
dc.contributor.author	Lenz, Martin
dc.contributor.author	Broedersz, Chase
dc.contributor.author	Guo, Ming
dc.contributor.corporatename	Georgia Institute of Technology. Center for the Science and Technology of Advanced Materials and Interfaces	en_US
dc.contributor.corporatename	Princeton University	en_US
dc.date.accessioned	2018-06-05T17:11:29Z
dc.date.available	2018-06-05T17:11:29Z
dc.date.issued	2018-04-19
dc.description	Presented at the Symposium on Soft Matter Forefronts "Contributed Talks", April 19, 2018, from 2:00 p.m.-2:50 p.m. at the Marcus Nanotechnology Building, Rooms 1116-1118, Georgia Tech.	en_US
dc.description	Chairs: Kazem Edmond (Exxon) & Alex Alexeev (Georgia Tech).	en_US
dc.description	Pierre Ronceray is with Princeton University.	en_US
dc.description	Runtime: 11:44 minutes	en_US
dc.description.abstract	Animal cells in tissues are supported by biopolymer matrices, which exhibit highly nonlinear mechanical properties. Here we show that this nonlinearity allows living contractile cells to generate a massive stiffness gradient in three distinct 3D extracellular matrix model systems: collagen, fibrin, and Matrigel. We decipher this remarkable behavior by introducing Nonlinear Stress Inference Microscopy (NSIM), a novel technique to infer stress fields in a 3D matrix from nonlinear microrheology measurement with optical tweezers. Using NSIM and simulations, we reveal a long-ranged propagation of cell-generated stresses resulting from local filament buckling. This slow decay of stress gives rise to the large spatial extent of the observed cell-induced matrix stiffness gradient, which could form a mechanism for mechanical communication between cells.	en_US
dc.description.sponsorship	Georgia Institute of Technology. College of Sciences	en_US
dc.description.sponsorship	Georgia Institute of Technology. Institute for Materials	en_US
dc.description.sponsorship	Georgia Institute of Technology. Parker H. Petit Institute for Bioengineering and Bioscience	en_US
dc.description.sponsorship	Georgia Institute of Technology. School of Materials Science and Engineering	en_US
dc.description.sponsorship	Georgia Institute of Technology. School of Physics	en_US
dc.description.sponsorship	American Physical Society	en_US
dc.description.sponsorship	Exxon Mobil Corporation	en_US
dc.description.sponsorship	National Science Foundation (U.S.)	en_US
dc.format.extent	11:44 minutes
dc.identifier.uri	http://hdl.handle.net/1853/59986
dc.language.iso	en_US	en_US
dc.publisher	Georgia Institute of Technology	en_US
dc.subject	Cell-generated stresses	en_US
dc.subject	Local filament buckling	en_US
dc.subject	Nonlinear stress inference microscopy	en_US
dc.subject	Nonlinearity	en_US
dc.subject	Soft matter	en_US
dc.subject	Stiffness gradient	en_US
dc.subject	Stress fields	en_US
dc.title	Cell Contraction Induces Long-Ranged Stress Stiffening in the Extracellular Matrix	en_US
dc.type	Moving Image
dc.type.genre	Lecture
dspace.entity.type	Publication
local.contributor.corporatename	Soft Matter Incubator
local.contributor.corporatename	Center for the Science and Technology of Advanced Materials and Interfaces
relation.isOrgUnitOfPublication	95867400-60a4-4b13-be33-8c9ea9434266
relation.isOrgUnitOfPublication	a21b130a-9b72-4c0c-b82d-22f981aa1d12