Title:
Electrospun Nanofibrous Patches for the Delivery of Cardiac-Derived c-Kit+ Cells
Electrospun Nanofibrous Patches for the Delivery of Cardiac-Derived c-Kit+ Cells
Author(s)
Streeter, Benjamin William
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Abstract
Congenital heart disease (CHD) affects 8 in 1000 live births and is the number one cause of birth defect-related deaths. Current treatment is surgical correction of the abnormality, but these surgeries are often only palliative and lead to right ventricular heart failure (RVHF). One promising avenue to restore the contractile function of the right ventricle is cardiac cell therapy. However, cell injection is hampered by low cell retention and survival following injection into the heart. Additionally, autologous cell therapy for CHD may suffer from patient-to-patient variability in cell efficacy. To combat these issues, therapeutic cells can be combined with biomaterials that serve as a delivery vehicle to enhance cell survival and modulate reparative paracrine release in vivo. The aim of this dissertation is to combine electrospun nanofibrous polycaprolactone (PCL) scaffolds and cardiac-derived c-Kit+ cell (CPCs) to create an epicardial cardiac patch. We investigate the effects of PCL fiber alignment and inclusion of extracellular matrix (ECM)-mimicking adhesion factors gelatin and fibronectin on CPCs from different donors and develop patient-specific cardiac patches capable of restoring RV function in a rat model of RVHF.
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Date Issued
2021-12-06
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Dissertation