Title:
Biomaterials for tissue engineering for rheumatoid arthritis based on controlling dendritic cell phenotype

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dc.contributor.advisor Babensee, Julia E.
dc.contributor.author Park, Jaehyung en_US
dc.contributor.committeeMember Boyan, Barbara
dc.contributor.committeeMember Garcia, Anes
dc.contributor.committeeMember Guldberg, Robert
dc.contributor.committeeMember McDevitt, Todd
dc.contributor.department Biomedical Engineering en_US
dc.date.accessioned 2012-01-10T20:48:52Z
dc.date.available 2012-01-10T20:48:52Z
dc.date.issued 2009-06-09 en_US
dc.description.abstract The host response toward biomaterial component of tissue-engineered devices has been extensively investigated. The objective of this research was to understand the response of dendritic cells (DCs) to different biomaterials upon contact and identify biomaterials suitable for use in tissue engineering constructs for rheumatoid arthritis (RA) applications. Differential levels of functional DC maturation were observed depending on the type of biomaterial in 2-dimensional films or 3-dimensional scaffolds used to treat immature DCs; Poly(lactic-co-glycolic acid) (PLGA) or chitosan supported higher levels of DC maturation, as compared to immature DCs. Alginate supported moderate levels of DC maturation. Agarose did not support DC maturation whereas hyaluronic acid inhibited DC maturation. Further, these DCs treated with different biomaterials induced differential phenotype and polarization of autologous T cells upon co-culture of DCs and T cells; DCs treated with PLGA induced T helper type I with immunogenic response while DCs treated with agarose did T helper type II with tolerogenic response. Effect of different biomaterials (PLGA and agarose) was assessed in vivo upon implantation of them into the knee joint of RA-induced rabbit. Total leukocyte concentrations in the peripheral blood or in the joint lavage of the left knees (untreated control) were observed in differential levels depending on the biomaterial implant, possibly due to the systemic circulation of the peripheral blood. Furthermore, cartilage and bone healing progression was differentially observed in the osteochondral defect of the knee joint of RA-induced rabbit, depending on type of biomaterial scaffold implanted into the defect. Collectively, these results demonstrate the multifunctional impacts of inherently different biomaterials on in vitro immunomodulation of phenotype and polarization of DCs and autologous T cells. Furthermore, taken together with these immunomodulatory impacts of biomaterials, in vivo effects of different biomaterial scaffolds on RA environment shown in this study can suggest the criteria of selection and design of biomaterials for orthopedic tissue engineering, which may ultimately be best integrated into the diseased cartilage and bone. en_US
dc.description.degree Ph.D. en_US
dc.identifier.uri http://hdl.handle.net/1853/42174
dc.publisher Georgia Institute of Technology en_US
dc.subject Immune response en_US
dc.subject Phenotype en_US
dc.subject Inflammatory en_US
dc.subject Tissue engineering en_US
dc.subject Adaptive immunity en_US
dc.subject Host response en_US
dc.subject Dendritic cell en_US
dc.subject Rheumatoid arthritis en_US
dc.subject Biomaterial en_US
dc.subject.lcsh Tissue engineering
dc.subject.lcsh Biomedical materials
dc.subject.lcsh Dendritic cells
dc.subject.lcsh Immune response
dc.subject.lcsh Rheumatoid arthritis
dc.title Biomaterials for tissue engineering for rheumatoid arthritis based on controlling dendritic cell phenotype en_US
dc.type Text
dc.type.genre Dissertation
dspace.entity.type Publication
local.contributor.advisor Babensee, Julia E.
local.contributor.corporatename Wallace H. Coulter Department of Biomedical Engineering
local.contributor.corporatename College of Engineering
relation.isAdvisorOfPublication 3dd0e95d-8545-4226-a030-faa5712fb312
relation.isOrgUnitOfPublication da59be3c-3d0a-41da-91b9-ebe2ecc83b66
relation.isOrgUnitOfPublication 7c022d60-21d5-497c-b552-95e489a06569
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