Title:
Predicted Functional RNAs within Coding Regions Constrain Evolutionary Rates of Yeast Proteins
Predicted Functional RNAs within Coding Regions Constrain Evolutionary Rates of Yeast Proteins
| dc.contributor.author | Warden, Charles David | en_US |
| dc.contributor.author | Kim, Seong-Ho | en_US |
| dc.contributor.author | Yi, Soojin V. | en_US |
| dc.contributor.corporatename | Georgia Institute of Technology. School of Biology | en_US |
| dc.contributor.corporatename | Indiana University School of Medicine. Division of Biostatistics | en_US |
| dc.date.accessioned | 2009-12-11T20:20:32Z | |
| dc.date.available | 2009-12-11T20:20:32Z | |
| dc.date.issued | 2008-02-13 | |
| dc.description | © 2008 Warden et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en |
| dc.description | DOI: 10.1371/journal.pone.0001559 | |
| dc.description.abstract | Functional RNAs (fRNAs) are being recognized as an important regulatory component in biological processes. Interestingly, recent computational studies suggest that the number and biological significance of functional RNAs within coding regions (coding fRNAs) may have been underestimated. We hypothesized that such coding fRNAs will impose additional constraint on sequence evolution because the DNA primary sequence has to simultaneously code for functional RNA secondary structures on the messenger RNA in addition to the amino acid codons for the protein sequence. To test this prediction, we first utilized computational methods to predict conserved fRNA secondary structures within multiple species alignments of Saccharomyces sensu strico genomes. We predict that as much as 5% of the genes in the yeast genome contain at least one functional RNA secondary structure within their protein-coding region. We then analyzed the impact of coding fRNAs on the evolutionary rate of protein-coding genes because a decrease in evolutionary rate implies constraint due to biological functionality. We found that our predicted coding fRNAs have a significant influence on evolutionary rates (especially at synonymous sites), independent of other functional measures. Thus, coding fRNA may play a role on sequence evolution. Given that coding regions of humans and flies contain many more predicted coding fRNAs than yeast, the impact of coding fRNAs on sequence evolution may be substantial in genomes of higher eukaryotes. | en |
| dc.identifier.citation | Warden CD, Kim S-H, Yi SV (2008) Predicted Functional RNAs within Coding Regions Constrain Evolutionary Rates of Yeast Proteins. PLoS ONE 3(2): e1559. doi:10.1371/journal.pone.0001559 | en |
| dc.identifier.doi | 10.1371/journal.pone.0001559 | |
| dc.identifier.issn | 1932-6203 | |
| dc.identifier.uri | http://hdl.handle.net/1853/31379 | |
| dc.language.iso | en_US | en |
| dc.publisher | Georgia Institute of Technology | en |
| dc.publisher.original | Public Library of Science | |
| dc.subject | Functional RNA | en |
| dc.subject | Conserved fRNA secondary structures | en |
| dc.subject | Evolutionary rate of protein-coding genes | en |
| dc.subject | Eukaryotes | en |
| dc.title | Predicted Functional RNAs within Coding Regions Constrain Evolutionary Rates of Yeast Proteins | en |
| dc.type | Text | |
| dc.type.genre | Article | |
| dspace.entity.type | Publication | |
| local.contributor.corporatename | College of Sciences | |
| local.contributor.corporatename | School of Biological Sciences | |
| relation.isOrgUnitOfPublication | 85042be6-2d68-4e07-b384-e1f908fae48a | |
| relation.isOrgUnitOfPublication | c8b3bd08-9989-40d3-afe3-e0ad8d5c72b5 |