Title:
Conversion of Stable RNA Hairpin to a Metastable Dimer in Frozen Solution
Conversion of Stable RNA Hairpin to a Metastable Dimer in Frozen Solution
| dc.contributor.author | Sun, Xueguang | en_US |
| dc.contributor.author | Li, J. Michael | en_US |
| dc.contributor.author | Wartell, Roger M. | en_US |
| dc.contributor.corporatename | Georgia Institute of Technology. School of Biology | en_US |
| dc.date.accessioned | 2013-10-07T18:55:27Z | |
| dc.date.available | 2013-10-07T18:55:27Z | |
| dc.date.issued | 2007-12 | |
| dc.description | ©2007 by Cold Spring Harbor Laboratory | en_US |
| dc.description | DOI: 10.1261/rna.433307 | en_US |
| dc.description.abstract | Previous studies employing a 79-nucleotide (nt) RNA indicated that this RNA could form two bands in a native polyacrylamide gel while one band was observed in a denaturing gel. This report describes an investigation on the nature of the two corresponding structures and the segment responsible for forming the slower mobility band. Sedimentation equilibrium of the 79-nt RNA was consistent with the two gel bands corresponding to monomer and dimer forms. The portion of the RNA required for dimer formation was explored using a secondary structure prediction algorithm of two 79-nt RNAs linked in a head-to-tail fashion. The predicted structure suggested that the first 21-nt at the 59 end of each RNA formed a self complementary duplex. A ribonuclease H assay carried out with RNA prepared as monomer (M), or a mixture of monomer and dimer (M/D), gave results consistent with the predicted M and D structures. Gel mobility experiments on 59 and 39 segments of the 79-nt RNA also indicated that dimer formation was due to the 21-nt 59 end. Studies on the 21-nt RNA molecule and sequence variants showed that this sequence can form a hairpin and a dimer complex. Unexpectedly, the hairpin to dimer conversion was shown to occur at high efficiency in frozen solution, although little or no conversion was observed above 0°C. The results indicate that a freezing environment can promote formation of intermolecular RNA complexes from stable RNA hairpins, supporting the notion that this environment could have played a role in the evolution of RNA complexity. | en_US |
| dc.identifier.citation | Xueguang Sun, J. Michael Li, and Roger M. Wartell, “Conversion of Stable RNA Hairpin to a Metastable Dimer in Frozen Solution,” RNA December 2007 13: 2277-2286. | en_US |
| dc.identifier.doi | 10.1261/rna.433307 | |
| dc.identifier.issn | 1355-8382 | |
| dc.identifier.uri | http://hdl.handle.net/1853/49188 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Georgia Institute of Technology | en_US |
| dc.publisher.original | Cold Spring Harbor Laboratory Press | en_US |
| dc.subject | RNA dimerization | en_US |
| dc.subject | Frozen solution RNA folding | en_US |
| dc.title | Conversion of Stable RNA Hairpin to a Metastable Dimer in Frozen Solution | en_US |
| dc.type | Text | |
| dc.type.genre | Article | |
| dspace.entity.type | Publication | |
| local.contributor.author | Wartell, Roger M. | |
| local.contributor.corporatename | College of Sciences | |
| local.contributor.corporatename | School of Biological Sciences | |
| relation.isAuthorOfPublication | 94639a95-f848-4076-af19-c0e13509ad24 | |
| relation.isOrgUnitOfPublication | 85042be6-2d68-4e07-b384-e1f908fae48a | |
| relation.isOrgUnitOfPublication | c8b3bd08-9989-40d3-afe3-e0ad8d5c72b5 |
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