Investigating effect of oxidative stress during in vitro myogenic differentiation

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Kang, Innie
Jang, Young C.
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Stem cell research has been developing new ways to understand the mechanism behind aging in muscles as well as ways to prevent debilitating symptoms of it. In this study, Pax7-CreER-Sod1 floxed cells were used to model aging. 4-Hydroxy-Tamoxifen was used to activate the knockout mechanism in the floxed cells. The absence, or knockout, of the Sod1 gene contributes to the inability to break down reactive oxidative species. In vitro, the oxidative stress on the culture not only affects proliferation, but also affects differentiation of myoblasts. Increased 4-OHT concentration led to decreased nuclei quantity, myotube formation, and fusion index. While research linking “young” and “aged” circulatory systems has been done in vivo, this research aims to understand the fusion of “aged” and “young” cells in vitro. The breakdown of oxidative species is important for increased cell division, myotubule formation, and muscle fiber formation. More research must be conducted to identify the mechanism behind this behavior such as quantifying reduced oxidative stress with the contribution of Sod1-expressing cells in Sod1-null cultures.
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Undergraduate Thesis
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