Control of Biofilm Bacteria

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Elder, Elisabeth D.
Fenech, Amy S.
Zink, Amy D.
Bradley, T. Paige
Fullers, A. Lynn
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Based on problems associated with the use of chlorine as a water disinfectant and with the control of bacterial populations in biofilms, 1,3-dichloro-2,2,5,5-tetramethy1-4- imidazolidinone (compound DC) and 1-bromo-3-chloro-2,2,5,5- tetramethy1-4-imidazolidinone (compound DBC) were used to control planktonic and sessile populations of Klebsiella. Following the suspension of stainless steel disks in tubes, the tubes were capped with gauze/cotton plugs and autoclaved. Sterile chlorine demand-free buffers and inocula of mucoid and nonmucoid variants of Klebsiella were introduced to the tubes to initiate biofilm formation. After 24 to 48 hours equilibration time, both sessile and planktonic cells were exposed to varying concentrations of the disinfectants. Fluid samples and disk surface scrapings were used to determine the length of time required to achieve a 99.9999% decrease in viable cells. While disinfection rates were slower with the sessile organisms than with the planktonic organisms, both organic N-halamine disinfectants achieved 6 log decreases in viable Klebsiella in less than 12 hours.
Sponsored and Organized by: U.S. Geological Survey, Georgia Department of Natural Resources, The University of Georgia, Georgia State University, Georgia Institute of Technology
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