Title:
Control of Biofilm Bacteria
Control of Biofilm Bacteria
Author(s)
Elder, Elisabeth D.
Fenech, Amy S.
Zink, Amy D.
Bradley, T. Paige
Fullers, A. Lynn
Fenech, Amy S.
Zink, Amy D.
Bradley, T. Paige
Fullers, A. Lynn
Advisor(s)
Editor(s)
Hatcher, Kathryn J.
Collections
Supplementary to
Permanent Link
Abstract
Based on problems associated with the use of
chlorine as a water disinfectant and with the control of bacterial
populations in biofilms, 1,3-dichloro-2,2,5,5-tetramethy1-4-
imidazolidinone (compound DC) and 1-bromo-3-chloro-2,2,5,5-
tetramethy1-4-imidazolidinone (compound DBC) were used to
control planktonic and sessile populations of Klebsiella.
Following the suspension of stainless steel disks in tubes, the
tubes were capped with gauze/cotton plugs and autoclaved.
Sterile chlorine demand-free buffers and inocula of mucoid and
nonmucoid variants of Klebsiella were introduced to the tubes to
initiate biofilm formation. After 24 to 48 hours equilibration
time, both sessile and planktonic cells were exposed to varying
concentrations of the disinfectants. Fluid samples and disk
surface scrapings were used to determine the length of time
required to achieve a 99.9999% decrease in viable cells. While
disinfection rates were slower with the sessile organisms than
with the planktonic organisms, both organic N-halamine
disinfectants achieved 6 log decreases in viable Klebsiella in less
than 12 hours.
Sponsor
Sponsored and Organized by: U.S. Geological Survey, Georgia Department of Natural Resources, The University of Georgia, Georgia State University, Georgia Institute of Technology
Date Issued
1995-04
Extent
Resource Type
Text
Resource Subtype
Proceedings