Title:
Caveolae and Caveolin-1 are important for Vitamin D signalling

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dc.contributor.advisor Boyan, Barbara D.
dc.contributor.author Wong, Kevin L. en_US
dc.contributor.committeeMember Jo, Hanjoong
dc.contributor.committeeMember Rubin, Janet
dc.contributor.committeeMember Schwartz, Zvi
dc.contributor.department Biomedical Engineering en_US
dc.date.accessioned 2011-03-04T20:06:01Z
dc.date.available 2011-03-04T20:06:01Z
dc.date.issued 2010-10-20 en_US
dc.description.abstract The most active form of Vitamin D, 1alpha,25(OH)2D3, modulates cells via receptor mediated mechanisms. While studies have elucidated the pathway via the classical nuclear Vitamin D Receptor (VDR), little is known about the membrane-associated Vitamin D Receptor (ERp60). Caveolae and its characteristic protein Caveolin-1 have been involved in many signaling pathways due to its specific structure and physical configuration. Other studies have shown that many components of the Vitamin D pathway have been found in caveolae. This study hypothesizes that caveolae and Caveolin-1 are important for the effects of 1,25 Vitamin D signaling via ERp60. Research up to date have shown that in rat and mouse growth zone chondrocytes, cells deprived of intact caveolae either through disruption through beta-Cyclodextrin or genetic knockout do not exhibit the characteristic responses to Vitamin D through ERp60 when compared to chondrocytes with functional caveolae. Studies using immunofluorescence co-localization and caveolae fractionation have shown that ERp60 is localized in the caveolae domains. Cellular fractionation was also performed to examine the localization of the ERp60 receptor in lipid rafts and caveolae. Histology and transmission electron microscopy were also used to examine the physiological importance of caveolae and Caveolin-1 in growth plate morphology and cellular characteristics. en_US
dc.description.degree M.S. en_US
dc.identifier.uri http://hdl.handle.net/1853/37086
dc.publisher Georgia Institute of Technology en_US
dc.subject Chondrocytes en_US
dc.subject Lipid raft en_US
dc.subject Cav-1 knockout mice en_US
dc.subject Matrix vesicles en_US
dc.subject Protein kinase C en_US
dc.subject.lcsh Cartilage cells
dc.subject.lcsh Human physiology
dc.subject.lcsh Cholecalciferol
dc.subject.lcsh Vitamin D
dc.title Caveolae and Caveolin-1 are important for Vitamin D signalling en_US
dc.type Text
dc.type.genre Thesis
dspace.entity.type Publication
local.contributor.corporatename Wallace H. Coulter Department of Biomedical Engineering
local.contributor.corporatename College of Engineering
relation.isOrgUnitOfPublication da59be3c-3d0a-41da-91b9-ebe2ecc83b66
relation.isOrgUnitOfPublication 7c022d60-21d5-497c-b552-95e489a06569
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