Title:
Enhancement of Ankle Fusion through FK506 Induced Osteogenesis
Enhancement of Ankle Fusion through FK506 Induced Osteogenesis
Author(s)
Huffman, Nicholas
Advisor(s)
Patel, Jay
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Abstract
Ankle Arthrodesis is a common surgical procedure that typically involves the fusion of the tibia and talus of the patient. During surgery, the surgeon uses screws and plates to compress the bones together and cease plantar and dorsiflexion motion [1]. However, one of the main complications with the surgery is the non-union of bones. This can be due to loosening of the screws or failure to grow new bone in the joint space. Our team hypothesized that introducing an additional orthobiologic into the system would assist in bone formation and reducing non-union rates. In this study, we evaluated the effectiveness of osteogenic drugs to improve bone fusion within ankle arthrodesis. One such molecule we evaluated is FK506 (Tacrolimus), an FDA approved drug for treating organ transplant rejection. We implemented a cell culture model to test out the osteogenic potential of FK506. Bovine Marrow Derived Cells (MDCs) were cultured for 1-2 weeks and evaluated with Alizarin Red S Staining, Results were also tested with hMSCs. ALP Activity, and Gene Expression. We found that FK506 significantly affects Alizarin Red S staining within our MDCs. Additionally, we identified that rhPDGF-bb could be a potential adjuvant to FK506 treatment. Though future work will be needed to confirm the effects of rhPDGF-bb within an in vivo model. It was also noticed that there was significant variation associated with the MDC results between donors. We will look to answer those questions with flow cytometry in future experiments. Following those results, we tested our model within a rabbit ankle model to evaluate effectiveness.
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Date Issued
2023-12-14
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