Title:
Flow-enhanced adhesion regulated by a selectin interdomain hinge
Flow-enhanced adhesion regulated by a selectin interdomain hinge
| dc.contributor.author | Lou, Jizhong | en_US |
| dc.contributor.author | Yago, Tadayuki | en_US |
| dc.contributor.author | Klopocki, Arkadiusz G. | en_US |
| dc.contributor.author | Mehta, Padmaja | en_US |
| dc.contributor.author | Chen, Wei | en_US |
| dc.contributor.author | Zarnitsyna, Veronika I. | en_US |
| dc.contributor.author | Bovin, Nicolai V. | en_US |
| dc.contributor.author | Zhu, Cheng | en_US |
| dc.contributor.author | McEver, Rodger P. | en_US |
| dc.contributor.corporatename | Oklahoma Medical Research Foundation. Cardiovascular Biology Research Program | en_US |
| dc.contributor.corporatename | University of Oklahoma Health Sciences Center. Dept. of Biochemistry and Molecular Biology | en_US |
| dc.contributor.corporatename | Georgia Institute of Technology. Institute for Bioengineering and Bioscience | en_US |
| dc.contributor.corporatename | Georgia Institute of Technology. Dept. of Biomedical Engineering | en_US |
| dc.contributor.corporatename | Emory University. Dept. of Biomedical Engineering | en_US |
| dc.contributor.corporatename | Georgia Institute of Technology. School of Mechanical Engineering | en_US |
| dc.contributor.corporatename | Institut bioorganicheskoĭ khimii im. M.M. Shemi︠a︡kina i I︠U︡. A. Ovchinnikova | en_US |
| dc.date.accessioned | 2013-06-03T20:16:36Z | |
| dc.date.available | 2013-06-03T20:16:36Z | |
| dc.date.issued | 2006-09 | |
| dc.description | © 2006 The Authors | en_US |
| dc.description | The electronic version of this article is the complete one and can be found online at: http://www.jcb.org/cgi/doi/10.1083/jcb.200606056 | en_US |
| dc.description | DOI: 10.1083/jcb.200606056 | en_US |
| dc.description.abstract | L-selectin requires a threshold shear to enable leukocytes to tether to and roll on vascular surfaces. Transport mechanisms govern flow-enhanced tethering, whereas force governs fl ow-enhanced rolling by prolonging the lifetimes of L-selectin–ligand complexes (catch bonds). Using selectin crystal structures, molecular dynamics simulations, site-directed mutagenesis, single-molecule force and kinetics experiments, Monte Carlo modeling, and flow chamber adhesion studies, we show that eliminating a hydrogen bond to increase the fl exibility of an interdomain hinge in L-selectin reduced the shear threshold for adhesion via two mechanisms. One affects the on-rate by increasing tethering through greater rotational diffusion. The other affects the off-rate by strengthening rolling through augmented catch bonds with longer lifetimes at smaller forces. By forcing open the hinge angle, ligand may slide across its interface with L-selectin to promote rebinding, thereby providing a mechanism for catch bonds. Thus, allosteric changes remote from the ligand-binding interface regulate both bond formation and dissociation. | en_US |
| dc.identifier.citation | Lou J., Yago T., Klopocki A.G., Mehta P., Chen W., Zarnitsyna V.I., Bovin N.V., Zhu C., McEver R.P. "Flow-enhanced adhesion regulated by a selectin interdomain hinge," Journal of cell biology, 174, 7, 1107-1117 (September 25 2006) | en_US |
| dc.identifier.doi | 10.1083/jcb.200606056 | |
| dc.identifier.issn | 0021-9525 | |
| dc.identifier.uri | http://hdl.handle.net/1853/47160 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Georgia Institute of Technology | en_US |
| dc.publisher.original | Rockefeller University Press | en_US |
| dc.subject | Interdomain hinge | en_US |
| dc.subject | Ligands | en_US |
| dc.subject | L-selectin | en_US |
| dc.subject | Monte Carlo simulations | en_US |
| dc.subject | P-selectin | en_US |
| dc.subject | Shear threshold | en_US |
| dc.subject | Adhesion | en_US |
| dc.title | Flow-enhanced adhesion regulated by a selectin interdomain hinge | en_US |
| dc.type | Text | |
| dc.type.genre | Article | |
| dspace.entity.type | Publication | |
| local.contributor.author | Zhu, Cheng | |
| local.contributor.corporatename | Wallace H. Coulter Department of Biomedical Engineering | |
| local.contributor.corporatename | College of Engineering | |
| relation.isAuthorOfPublication | d28f1a84-f07d-40ec-bed3-60bc4c140551 | |
| relation.isOrgUnitOfPublication | da59be3c-3d0a-41da-91b9-ebe2ecc83b66 | |
| relation.isOrgUnitOfPublication | 7c022d60-21d5-497c-b552-95e489a06569 |
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