Title:
Two dimensional (solid phase) kinetic analysis of FCnGamma receptor III (CD16) Interactions with IgG

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dc.contributor.advisor Zhu, Cheng
dc.contributor.advisor Selvaraj, Periasamy
dc.contributor.author Chesla, Scott Edward en_US
dc.contributor.committeeMember Lyle Sinor
dc.contributor.committeeMember Vito, Raymond P.
dc.contributor.committeeMember Nerem, Robert M.
dc.contributor.committeeMember Wick, Timothy M.
dc.contributor.department Mechanical Engineering en_US
dc.date.accessioned 2005-09-16T15:49:49Z
dc.date.available 2005-09-16T15:49:49Z
dc.date.issued 2005-06-06 en_US
dc.description.abstract Cellular adhesion research has recently focused on the small scale at the level of individual receptor-ligand bonds. This trend in research is primarily due to experimental advances which allow such individual bond force measurements. Here, one of these techniques, micromanipulation, has been extended to not only determine the bond force of individual receptor-ligand pairs, but also the intrinsic kinetic rates of the interaction. Using transmembrane (TM ) Fc gamma receptor III (CD16a-TM) and human IgG (hIgG), the dependence of adhesion probability on receptor-ligand expression densities, contract duration and contact area was quantitated. A probabilistic based theoretical formulation was developed and validated that relates the intrinsic molecular kinetic rates of the receptorVligand interaction to the experimentally determined adhesion probability. This theoretical formulation describing individual receptor-ligand kinetics has also allowed direct evaluation of existing biophysical bond strength/kinetics paradigms at the extreme condition of single bonds. A force-displacement model was also developed to quantitate the force exerted on the RBC membrane transducer during the micropipette retraction process and found to be in agreement with previous work. In addition to CD16a-TM, the kinetic rates of CD16a anchored via a glycosyl phosphatidylinositol (GPI) moiety (CD16a-GPI) and the two alleles of CD16b (NA1 and NA2) were determined for human, rabbit, and mouse IgG species. The binding affinity of these CD16 interactions to soluble IgG was also measured by traditional bulk chemistry approaches and compared to those measured via the micromanipulation protocol in which the IgG ligand is membrane bound in the solid phase. These data suggest that the membrane anchor itself can alter CD16 binding properties. This represents the first reported effect of the anchor on an intrinsic receptor property, its kinetic rates and binding affinity. This thesis presents two specific aims or goals. These goals were achieved and reported in this thesis. During the course of this research, I also explored other directions and gathered initial data. These directions were further explored by other researchers but the initial data is also presented here. en_US
dc.description.degree Ph.D. en_US
dc.format.extent 6142702 bytes
dc.format.mimetype application/pdf
dc.identifier.uri http://hdl.handle.net/1853/7276
dc.language.iso en_US
dc.publisher Georgia Institute of Technology en_US
dc.subject CD16
dc.subject Cell receptors
dc.subject IgG
dc.subject Immunoglobulin G.
dc.subject Kinetic analysis
dc.subject 2D
dc.subject Two-dimensional solid
dc.title Two dimensional (solid phase) kinetic analysis of FCnGamma receptor III (CD16) Interactions with IgG en_US
dc.type Text
dc.type.genre Dissertation
dspace.entity.type Publication
local.contributor.advisor Zhu, Cheng
local.contributor.corporatename George W. Woodruff School of Mechanical Engineering
local.contributor.corporatename College of Engineering
relation.isAdvisorOfPublication d28f1a84-f07d-40ec-bed3-60bc4c140551
relation.isOrgUnitOfPublication c01ff908-c25f-439b-bf10-a074ed886bb7
relation.isOrgUnitOfPublication 7c022d60-21d5-497c-b552-95e489a06569
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