Title:
Mechanoregulation of chondrocytes and chondroprogenitors: the role of TGF-BETA and SMAD signaling

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dc.contributor.advisor Levenston, Marc E.
dc.contributor.author Mouw, Janna Kay en_US
dc.contributor.committeeMember García, Andrés J.
dc.contributor.committeeMember Boyan, Barbara
dc.contributor.committeeMember Christopher Jacobs
dc.contributor.committeeMember Harish Radhakrishna
dc.contributor.department Bioengineering en_US
dc.date.accessioned 2006-01-18T22:22:28Z
dc.date.available 2006-01-18T22:22:28Z
dc.date.issued 2005-11-28 en_US
dc.description.abstract In pathological states such as osteoarthritis, the complex metabolic balance of cartilage is disrupted, leading to a loss in the integrity and biomechanical function of cartilage. Osteoarthritis affects more than 20 million Americans, costing the United States economy over $60 billion yearly. Risk factors for osteoarthritis include age, excessive joint loading, and joint injury. Tissue engineering offers a potential solution for the replacement of diseased and/or damaged cartilage. Unfortunately, plentiful donor cell populations are difficult to assemble, as chondrocytes have a well characterized lack of expansion potential. Mesenchymal progenitor cells offer an alternative with a high expansion potential capable of supplying large quantities of cells. Using an immature bovine model, the chondrogenic differentiation of articular chondrocytes and bone marrow stromal cells was found to be scaffold, media and mechanical stimulation dependent. TGF-beta signaling participated in the response of articular chondrocytes to dynamic compressive loading, as well as enhanced the chondrogenesis of bovine BMSCs, through interactions between loading and TGF-beta/Smad signaling. Also, dynamic loading altered gene expression, matrix synthesis rates and intracellular phosphorylation for bovine BMSCs. However the response of the cells to dynamic loading depends on both media supplementation and the duration of unloaded culture. These studies establish signaling through the TGF-beta pathway as a mechanotransduction pathway for chondrocytes and chondroprogenitors in 3D culture. en_US
dc.description.degree Ph.D. en_US
dc.format.extent 1750505 bytes
dc.format.mimetype application/pdf
dc.identifier.uri http://hdl.handle.net/1853/7550
dc.language.iso en_US
dc.publisher Georgia Institute of Technology en_US
dc.subject Chondrocyte
dc.subject Stromal cells
dc.subject Chondrogenesis en_US
dc.subject.lcsh Cell differentiation en_US
dc.subject.lcsh Tissue engineering en_US
dc.subject.lcsh Articular cartilage en_US
dc.subject.lcsh Biomechanics en_US
dc.subject.lcsh Cartilage cells en_US
dc.title Mechanoregulation of chondrocytes and chondroprogenitors: the role of TGF-BETA and SMAD signaling en_US
dc.type Text
dc.type.genre Dissertation
dspace.entity.type Publication
local.contributor.corporatename College of Engineering
local.contributor.corporatename College of Engineering
local.relation.ispartofseries Doctor of Philosophy with a Major in Bioengineering
relation.isOrgUnitOfPublication 7c022d60-21d5-497c-b552-95e489a06569
relation.isOrgUnitOfPublication 7c022d60-21d5-497c-b552-95e489a06569
relation.isSeriesOfPublication 5db25cda-aa52-48d2-8f63-c551ef2c92f4
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