Title:
Detecting base pair substitutions in DNA fragments by temperature-gradient gel electrophoresis
Detecting base pair substitutions in DNA fragments by temperature-gradient gel electrophoresis
| dc.contributor.author | Wartell, Roger M. | en_US |
| dc.contributor.author | Hosseini, Seyed Homayoun | en_US |
| dc.contributor.author | Moran, Charles P., Jr. | en_US |
| dc.contributor.corporatename | Georgia Institute of Technology. School of Physics | en_US |
| dc.contributor.corporatename | Georgia Institute of Technology. School of Biology | en_US |
| dc.contributor.corporatename | Emory University. School of Medicine | en_US |
| dc.date.accessioned | 2010-10-27T18:05:21Z | |
| dc.date.available | 2010-10-27T18:05:21Z | |
| dc.date.issued | 1990-05-11 | |
| dc.description | © 1990 Oxford University Press. The definitive version can be found online at: http://nar.oxfordjournals.org/ | en_US |
| dc.description.abstract | A vertical gel electrophoresis apparatus is described which can distinguish DNA fragments differing by single base pair substitutions. The system employs a homogenous polyacrylamide gel containing ureaformamide and a temperature gradient which runs either perpendicular or parallel to the direction of electrophoresis. The temperature-gradient system simplifies several features of the denaturant-gradient system (1) and is relatively inexpensive to construct. Eight homologous 373 bp DNAs differing by one, two, or nine base pair substitutions were examined. DNA electrophoretic mobility changed abruptly with the temperature induced unwinding of DNA domains. GC to AT substitutions at different locations within the first melting domain, as well as an AT to TA transversion were separated with temperature gradients parallel to the electrophoretic direction. The relative stabilities of the DNAs observed in the gels were compared to predictions of DNA melting theory. General agreement was observed however complete correspondence was not obtained. | en_US |
| dc.identifier.citation | Roger M.Wartell, Seyed H.Hosseini and Charles P.Moran, Jr., "Detecting base pair substitutions in DNA fragments by temperature-gradient gel electrophoresis," Nucleic Acids Research, Vol. 18, No. 9 (May 11, 1990) 2699-2705. | en_US |
| dc.identifier.issn | 0305-1048 | |
| dc.identifier.uri | http://hdl.handle.net/1853/35615 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Georgia Institute of Technology | en_US |
| dc.publisher.original | Oxford University Press | |
| dc.subject | Gel electrophoresis | en_US |
| dc.subject | Temperature-gradient gel electrophoresis | en_US |
| dc.subject | Vertical temperature-gradient gel system | en_US |
| dc.subject | Polyacrylamide gel | en_US |
| dc.subject | DNA melting theory | en_US |
| dc.title | Detecting base pair substitutions in DNA fragments by temperature-gradient gel electrophoresis | en_US |
| dc.type | Text | |
| dc.type.genre | Article | |
| dspace.entity.type | Publication | |
| local.contributor.author | Wartell, Roger M. | |
| local.contributor.corporatename | College of Sciences | |
| local.contributor.corporatename | School of Biological Sciences | |
| relation.isAuthorOfPublication | 94639a95-f848-4076-af19-c0e13509ad24 | |
| relation.isOrgUnitOfPublication | 85042be6-2d68-4e07-b384-e1f908fae48a | |
| relation.isOrgUnitOfPublication | c8b3bd08-9989-40d3-afe3-e0ad8d5c72b5 |