Title:
Time-dependent signatures of acoustic wave biosensors
Time-dependent signatures of acoustic wave biosensors
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Author(s)
Hunt, William D.
Stubbs, Desmond D.
Lee, Sang-Hun
Stubbs, Desmond D.
Lee, Sang-Hun
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Abstract
Acoustic wave devices coated with a biolayer represent
one biosensor approach for the detection of medically relevant
biomolecules. In a typical application, the acoustic wave device
is connected in an oscillator circuit, and the frequency shift ∆ f
resulting from a biomolecular event is recorded. In this paper, we
discuss our recent work in this field, which has included the use
of Rayleigh wave surface acoustic wave devices for vapor phase
detection as well as quartz crystal microbalance devices for liquid
phase measurements. For all of the results reported herein the
biofilm on the surface of the acoustic wave device consists of a
layer of antibodies raised against a specific target molecule or
antigen. We present our results for the vapor phase detection of
small molecules such as uranine and cocaine as well as liquid
phase detection of small and large molecules. The data we present
from these various experiments is the signature associated with the
biomolecular recognition events; that is, we record and present
∆ f(t). Finally, we present the recent results of our time-dependent
perturbation theory work, which gives a potential method for
resolving the acoustic wave biosensor signature into information
relating to molecular structure changes during a molecular
recognition event.
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Date Issued
2003-06
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