(Georgia Institute of Technology, 2002)
Pullman, Gerald S.; Johnson, Shannon
Loblolly pine (Pinus taeda L.) is one of the most important commercial trees in the U.S. To be successful for commercial use, somatic
embryogenesis technology must work with a variety of genetically diverse seeds. Initiation rates of loblolly pine were improved through a
combination of modified 1/2 P6 salts, activated carbon at 50–100 mgL–1, Cu and Zn added to compensate for adsorption by activated carbon,
1.5% maltose, 2% myo-inositol, (to raise osmotic level partially simulating the ovule environment), 500 mg L–1 case amino acids, 450 mg L–1
glutamine, 2 mg L–1 NAA, 0.45 mg L–1 BAP, 0.43 mg L–1 kinetin, and 1.6–2 g L–1 Gelrite. Across 10 open-pollinated families, initiation rates
ranged from 3–33%, averaging 16%.