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School of Biological Sciences

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Catabolite activator protein induced bending studied by covalent circularization of short DNA fragment

1985-12 , Dripps, David Joseph

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Ecology of juvenile white shrimp, Penaeus setiferus Linnaeus, in the salt marsh habitat

1985-05 , Mayer, Mary Anne

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A study of the Aerobic metabolism of Zymomonas mobilis

1984-12 , Stuff, Katharine Elizabeth

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Reproductive cycle and low salinity stress in adult Mercenaria mercenaria L of Warsaw Sound, Georgia

1984-05 , Pline, Marc Joseph

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The catabolite activator protein stabilizes its binding site in the E. coli lactose promoter

1985-10-25 , DeGrazia, Henry , Abhiraman, Saraswathy , Wartell, Roger M.

The effect of catabolite activator protein, CAP, on the thermal stability of DNA was examined. Site specific binding was studied with a 62 bp DNA restriction fragment containing the primary CAP site of the E. coli lactose (lac) promoter. A 144 bp DNA containing the lac promoter region and a 234 bp DNA from the pBR322 plasmid provided other DNA sites. Thermal denaturation of protein-DNA complexes was carried out in a low ionic strength solvent with 40% dimethyl sulfoxide, DMSO. In this solvent free DNA denatured below the denaturation temperature of CAP. The temperature stability of CAP for site specific binding was monitored using an acrylamide gel electrophoresis assay. Results show that both specific and non-specific CAP binding stabilize duplex DNA. Site specific binding to the 62 bp DNA produced a 13.3 degrees C increase in the transition under conditions where non-specific binding stabilized this DNA by 2-3 degrees C.

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Search for chemical stimuli that act on plant parasitic nematodes

1985 , Dusenbery, David Brock

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Measurements of community metabolism in barrier island beach runnels - Sapelo Island, Georgia

1984-12 , Vega, Nellie Enid

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The secondary production dynamics of simulium taxodium and simulium lakei (diptera: simuliidae) in the Ogeechee River, Georgia

1985-08 , Mizner, Jack H.

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Genetic specifications of nematode surface antigens

1985 , Politz, Samuel M.

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Functional morphology of intertidal seaweeds: adaptive significance of aggregate vs. solitary forms

1984-08-15 , Taylor, Phillip R. , Hay, Mark E.

Many intertidal seaweeds show a tremendous gradient of morphological form ranging from spatially separated thalli, to thalli that are aggregated into dense turfs. Aggregation of seaweeds into turfs decreases productivity per g organic weight due to crowding of thalli but increases resistance to desiccation. The intertidal distribution of the turf growth form is correlated with the intensity of desiccation stress. Also turfs transplanted into tide pools developed the non-turf morphology, while non-turfs transplanted to emergent substrate either developed the turf form or died. The turf growth form is energetically expensive; apparent productivity of turfs was 23 to 48 % less than that of individuals. Increasing light and nutrients available to turfs by separating the thalli increased apparent productivity by 36 to 113 %. We tested the hypothesis that these turfs minimize energetic costs of this aggregated growth form through the spatial partitioning of photosynthetic and respirative activity. The lower portions of turfs showed less apparent photosynthesis than the upper portions (reductions of 37 to 85 %); however, this spatial partitioning was also found in the individual forms (37 to 63 % reductions) of these relatively simple seaweeds. Spatial differentiation of respiration was similar in turfs and nonturfs. The seaweeds, examined are able to alter the extent of thalli compaction in accordance with varying levels of environmental stress. This phenotypic plasticity allows seaweeds to adopt morphological features that maximize fitness in a wide variety of habitats without being developmentally committed.

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