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Undergraduate Research Opportunities Program

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    Expanding test methods for marine ecotoxicology testing using Proales similis
    (Georgia Institute of Technology, 2020-05) Park, Nancy
    This study is the first inquiry into the suitability of the rotifer Proales similis as a test animal for researchers studying marine pollution. It also introduces a novel toxicity test based on inhibition of hatching rates of P. similis diapausing embryos. We exposed P. similis to various concentrations of cadmium, copper, and mercury and then quantified effects on survival, hatching, reproduction, and ingestion. We found that mortality and cyst hatching endpoints were more robust to heavy metal toxicity than reproduction or ingestion. Overall, we confirmed that P. similis is a suitable tool for convenient and quick ecotoxicology assessments, and we established cyst inhibition as a novel, reliable endpoint for observing effects of toxicity on rotifer populations.