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search.filters.applied.f.advisor: Cope, Timothy C. × Author: Palanivel, Shivani × End date: 2023 × Start date: 2020 × Item Type: Publication × search.filters.applied.f.isOrgUnitOfPublicationTitle: School of Biological Sciences ×

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Analysis of Neural Stem Cells and Neurospheres

2020-05 , Palanivel, Shivani

Neural stem cells (NSCs) are important multipotent adult stem cells capable of self-renewal and differentiation into various cell types present in the central nervous system. Studying the characteristics of NSCs and their differentiation offers insights into developmental mechanisms and allows us to assess their utility ​in vitro disease modeling and neurotoxicological research. This study investigates the properties of NSC during propagation and differentiation in culture. Neurospheres, formed from NSC aggregates, were cultured and examined for morphology and growth rate by cell counting and size analysis. Neurospheres were induced to differentiate toward neural network formation. The expression of markers of NSC and astrocytes in neurospheres during differentiation was detected by immunostaining methods. NSC multiplied with an exponential proliferation rate accompanied by a steady increase in neurosphere diameter. The neurosphere cells expressed Nestin, an NSC/ progenitor cell marker, when cultured in self-renewal media, and displayed pronounced GFAP expression after induction of differentiation indicating a prevalence of astrocytes. Further studies will provide better understanding of NSC characteristics that may be applied for the advancement of stem cell therapy to help combat neurodegenerative diseases.

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