Organizational Unit:
Undergraduate Research Opportunities Program

Permanent Link
https://hdl.handle.net/1853/70802
Research Organization Registry ID
Description
Previous Names
Parent Organization
Parent Organization
Organizational Unit
Includes Organization(s)
ArchiveSpace Name Record
https://finding-aids.library.gatech.edu/agents/corporate_entities/1180

Filters

1
2019 1
1
1
1
1
Reset filters

Settings
search.filters.applied.f.advisor: Agarwal, Vinayak × End date: 2019 × Start date: 2010 × search.filters.applied.f.isOrgUnitOfPublicationTitle: School of Chemistry and Biochemistry × search.filters.applied.f.isOrgUnitOfPublicationTitle: Undergraduate Research Opportunities Program ×

Publication Search Results

Now showing 1 - 1 of 1
Thumbnail Image
Item

Carrier protein and halogenase selectivity in the biosynthesis of halogenated pyrroles

2019-05 , Lail, Andrew J.

Natural product biosynthetic pathways often share similar architecture even when they lead to different final products. In polyketide synthase (PKS) and non-ribosomal peptide synthetase (NRPS) enzymatic pathways, the substrate is attached to a carrier protein (CP) while the tailoring enzymes make modifications to yield a final product. The CP may therefore have a role in determining what enzymes act on the substrate, influencing the final product’s chemistry. In this study, pyrrole halogenases from several different bacterial species were characterized in vitro to test their ability to halogenate pyrrolyl CPs from four different natural product biosynthetic pathways. The reactions were analyzed via mass spectrometry to determine the halogenation state of the products formed. This study concludes that only some halogenases can act promiscuously on CPs from other pathways. Additionally, there is some modulation in the number of halogenation events between certain CP and halogenase pairs. The selectivity of these halogenase and CP interactions is likely caused by protein-protein interactions, and the structure of the CP/halogenase complex may provide new insights into such interaction.

View