Title:
Sequence patterns and signatures: computational and experimental discovery of amyloid-forming peptides dataset
Sequence patterns and signatures: computational and experimental discovery of amyloid-forming peptides dataset
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Authors
Xiao, Xinqing
Robang, Alicia S.
Sarma, Sudeep
Le, Justin V.
Helmicki, Michael E.
Lambert, Matthew J.
Guerrero-Ferreira, Ricardo
Arboleda-Echavarria, Johana
Paravastu, Anant K.
Hall, Carol K.
Robang, Alicia S.
Sarma, Sudeep
Le, Justin V.
Helmicki, Michael E.
Lambert, Matthew J.
Guerrero-Ferreira, Ricardo
Arboleda-Echavarria, Johana
Paravastu, Anant K.
Hall, Carol K.
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Abstract
Screening amino acid sequence space via experiments to discover peptides that self-assemble into amyloid fibrils is challenging. We have developed a computational peptide assembly design (PepAD) algorithm, that enables the discovery of amyloid-forming peptides. Discontinuous molecular dynamics (DMD) simulation with the PRIME20 force field combined with the FoldAmyloid tool is used to examine the fibrilization kinetics of PepAD-generated peptides. PepAD screening of ∼10,000 7-mer peptides resulted in twelve top-scoring peptides with two distinct hydration properties. Our studies revealed that eight of the twelve in-silico discovered peptides spontaneously form amyloid fibrils in the DMD simulations and that all eight have at least five residues that the FoldAmyloid tool classifies as being aggregation-prone. Based on these observations, we re-examined the PepAD-generated peptides in the sequence pool returned by PepAD and extracted five sequence patterns as well as associated sequence signatures for the 7-mer amyloid-forming peptides. Experimental results from Fourier transform infrared spectroscopy (FTIR), thioflavin T (ThT) fluorescence, circular dichroism (CD), and transmission electron microscopy (TEM) indicate that all the peptides predicted to assemble in-silico assemble into antiparallel β-sheet nanofibers in a concentration-dependent manner. This is the first attempt to use a computational approach to search for amyloid-forming peptides based on customized settings. Our efforts facilitate the identification of β-sheet-based self-assembling peptides, and contribute insights towards answering a fundamental scientific question: “What does it take, sequence-wise, for a peptide to self-assemble?”
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Date Issued
2022-11
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