Title:
All Complete Bacterial and Archeal Genomes -- Set 6

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Author(s)
Konstantinidis, Kostas T.
Rodriguez, Luis M.
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Abstract
Motivation: Determining the fraction of the diversity within a microbial community sampled and the amount of sequencing required to cover the total diversity represent challenging issues for metagenomics studies. Due to these limitations, central ecological questions with respect to the global distribution of microbes and the functional diversity of their communities cannot be robustly assessed. Results: We introduce Nonpareil, a method to estimate and project coverage in metagenomes. Nonpareil does not rely on high-quality assemblies, OTU calling, or comprehensive reference databases; thus, it is broadly applicable to metagenomic studies. Application of Nonpareil on available metagenomic datasets provided estimates on the relative complexity of soil, freshwater and human microbiome communities, and suggested that about 200Gb of sequencing data are required for 95% abundance-weighted average coverage of the soil communities analyzed.
Sponsor
United States. Department of Energy
National Science Foundation (U.S.)
Date Issued
2014-02-03
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These data were collected from the National Center for Biotechnology Information (NCBI) database GenBank, which was designed to provide and encourage access within the scientific community to sources of current and comprehensive information. NCBI and Georgia Tech place no restrictions on the use or distribution of the data contained in this collection. However, some of the original data may be subject to patent, copyright, or other intellectual property rights. Neither NCBI nor Georgia Tech are in a position to assess the validity of such claims and since there is no transfer or rights from submitters to NCBI, NCBI has no rights to transfer to a third party. For more information on NCBI's copyright disclaimer, please see: http://www.ncbi.nlm.nih.gov/About/disclaimer.html
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