Title:
Integrative immunotranscriptomic analysis of long-lived plasma cells

dc.contributor.advisor Gibson, Greg
dc.contributor.advisor Lee, Eun-Hyung
dc.contributor.author Garimalla, Swetha N.
dc.contributor.committeeMember Yi, Soojin
dc.contributor.committeeMember Tirouvanziam, Rabin
dc.contributor.committeeMember Qiu, Peng
dc.contributor.department Biology
dc.date.accessioned 2020-05-20T16:47:55Z
dc.date.available 2020-05-20T16:47:55Z
dc.date.created 2019-05
dc.date.issued 2019-05-21
dc.date.submitted May 2019
dc.date.updated 2020-05-20T16:47:55Z
dc.description.abstract Long-lived plasma cells are a key component of serological memory encoded by the adaptive immune response. To date, prior studies of these cell types have largely assessed plasma cells as one homogenous population. The Lee Lab at Emory University provided strong evidence for the presence of subpopulations of plasma cells in the bone marrow and peripheral blood. In this work, I analyze six of these populations: pops 2, 3, and 5 in the peripheral blood and pops A, B, and D in the bone marrow sampled from live human donors. Whole-transcriptome analysis of these six flow-sorted plasma cell populations is compared within and across tissue within this study. Further, I present 4 possible models of development of these plasma cells based on the results. Finally, I performed a singlecell RNA sequencing analysis of the long-lived plasma cells (popD) and present evidence for the possibility of subpopulations of this cell type. This work describes many mechanisms of molecular development of long-lived plasma cells, some of which have been experimentally validated by Dr. Doan Nguyen as a result of these analyses, however, further experimental validation is required to validate all findings in this work.
dc.description.degree Ph.D.
dc.format.mimetype application/pdf
dc.identifier.uri http://hdl.handle.net/1853/62642
dc.language.iso en_US
dc.publisher Georgia Institute of Technology
dc.subject Plasma cells
dc.subject Antibody secreting cells
dc.title Integrative immunotranscriptomic analysis of long-lived plasma cells
dc.type Text
dc.type.genre Dissertation
dspace.entity.type Publication
local.contributor.advisor Gibson, Greg
local.contributor.corporatename College of Sciences
local.contributor.corporatename School of Biological Sciences
relation.isAdvisorOfPublication 5606ef18-bd5a-4b7b-b3fc-05821bf66602
relation.isOrgUnitOfPublication 85042be6-2d68-4e07-b384-e1f908fae48a
relation.isOrgUnitOfPublication c8b3bd08-9989-40d3-afe3-e0ad8d5c72b5
thesis.degree.level Doctoral
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