Identifying Functional Nuclear Receptor Signaling in Estrogen Receptor-Positive Breast Cancer

Mitchel, Jonathan Evan

Title:

Identifying Functional Nuclear Receptor Signaling in Estrogen Receptor-Positive Breast Cancer

dc.contributor.author	Mitchel, Jonathan Evan
dc.contributor.committeeMember	McDonald, John F.
dc.contributor.committeeMember	Skolnick, Jeffrey
dc.contributor.department	Biomedical Engineering (Joint GT/Emory Department)
dc.date.accessioned	2019-05-30T16:23:48Z
dc.date.available	2019-05-30T16:23:48Z
dc.date.created	2019-05
dc.date.issued	2019-05
dc.date.submitted	May 2019
dc.date.updated	2019-05-30T16:23:48Z
dc.description.abstract	We developed a microarray data analytical method capable of identifying ligand-activated nuclear receptors (NRs) in samples of the MCF7 cell line that are cultured among uncharacterized ligands. Principally, we applied this novel technique to reanalyze microarray data from an MCF7 xenograft experiment to reveal NR signaling that is likely the result of small molecule ligands available in the tumor microenvironment. Furthermore, this method was applied to query NR signaling caused by the small molecules present in fetal bovine serum to identify receptors stimulated by this common culture method. These analyses unveiled the potentially important roles of NR5A1 and NR1H4 in the progression of estrogen receptor-positive breast cancer. Preliminary experiments demonstrate that these receptors may play a role in mediating the transcriptional regulatory action of the estrogen receptor itself, potentially opening novel avenues for the development of more efficacious adjuvant therapies.
dc.description.degree	Undergraduate
dc.format.mimetype	application/pdf
dc.identifier.uri	http://hdl.handle.net/1853/61377
dc.language.iso	en_US
dc.publisher	Georgia Institute of Technology
dc.subject	Breast cancer
dc.subject	Nuclear receptors
dc.subject	Adjuvant therapy
dc.subject	Tumor microenvironment
dc.title	Identifying Functional Nuclear Receptor Signaling in Estrogen Receptor-Positive Breast Cancer
dc.type	Text
dc.type.genre	Undergraduate Thesis
dspace.entity.type	Publication
local.contributor.corporatename	Wallace H. Coulter Department of Biomedical Engineering
local.contributor.corporatename	Undergraduate Research Opportunities Program
local.contributor.corporatename	College of Engineering
local.relation.ispartofseries	Undergraduate Research Option Theses
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thesis.degree.level	Undergraduate