Hydrogel microchannel fabrication using laser microdissection technology
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Castro-Quilang, Kathryn
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Abstract
Lymphatic vasculature plays a critical role in immune system function, including a major role in facilitating transport of antigens and immune cells through the lymphatic fluid. While microfluidic chip models have been extensively used to study lymphatic vessels, using hydrogels to closely mimic in vivo environments by free-forming lymphatic network features and exploring patterned lymphatic networks, has seen limited research. This study investigates the potential of laser capture dissection technology, specifically utilizing photoablation settings, to create channels in microfluidic systems that emulate structural aspects of lymphatic vasculature. Through optimizations of hydrogel properties, microfluidic designs, and ZEISS PALM MicroBeam Laser Microdissection settings, well-defined patterned channels achieve flow and perfusion. The application of LCM technology holds significant promise for various lymphatic and vascular modeling applications, including tissue engineering, system or organ-on-chip platforms, and organoid models.
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2023-07-31
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