Title:
High-resolution mapping of h1 linker histone variants in embryonic stem cells.
High-resolution mapping of h1 linker histone variants in embryonic stem cells.
| dc.contributor.author | Cao, Kaixiang | en_US |
| dc.contributor.author | Lailler, Nathalie | en_US |
| dc.contributor.author | Zhang, Yunzhe | en_US |
| dc.contributor.author | Kumar, Ashwath | en_US |
| dc.contributor.author | Uppal, Karan | en_US |
| dc.contributor.author | Liu, Zheng | en_US |
| dc.contributor.author | Lee, Eva K. | en_US |
| dc.contributor.author | Wu, Hongwei | en_US |
| dc.contributor.author | Medrzycki, Magdalena | en_US |
| dc.contributor.author | Pan, Chenyi | en_US |
| dc.contributor.author | Ho, Po-Yi | en_US |
| dc.contributor.author | Cooper, Guy P. , Jr. | en_US |
| dc.contributor.author | Dong, Xiao | en_US |
| dc.contributor.author | Bock, Christoph | en_US |
| dc.contributor.author | Bouhassira, Eric E. | en_US |
| dc.contributor.author | Fan, Yuhong | en_US |
| dc.contributor.corporatename | Georgia Institute of Technology. School of Biology | en_US |
| dc.contributor.corporatename | Georgia Institute of Technology. Institute for Bioengineering and Bioscience | en_US |
| dc.contributor.corporatename | Albert Einstein College of Medicine. Dept. of Medicine | en_US |
| dc.contributor.corporatename | Georgia Institute of Technology. School of Industrial and Systems Engineering | en_US |
| dc.contributor.corporatename | Georgia Institute of Technology. School of Electrical and Computer Engineering | en_US |
| dc.contributor.corporatename | Research Center for Molecular Medicine of the Austrian Academy of Sciences | en_US |
| dc.contributor.corporatename | Medizinische Universität Wien | en_US |
| dc.contributor.corporatename | Max-Planck-Institut für Informatik | en_US |
| dc.date.accessioned | 2013-09-27T20:22:46Z | |
| dc.date.available | 2013-09-27T20:22:46Z | |
| dc.date.issued | 2013-04 | |
| dc.description | © 2013 Cao et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en_US |
| dc.description | DOI: 10.1371/journal.pgen.1003417. | en_US |
| dc.description.abstract | H1 linker histones facilitate higher-order chromatin folding and are essential for mammalian development. To achieve highresolution mapping of H1 variants H1d and H1c in embryonic stem cells (ESCs), we have established a knock-in system and shown that the N-terminally tagged H1 proteins are functionally interchangeable to their endogenous counterparts in vivo. H1d and H1c are depleted from GC- and gene-rich regions and active promoters, inversely correlated with H3K4me3, but positively correlated with H3K9me3 and associated with characteristic sequence features. Surprisingly, both H1d and H1c are significantly enriched at major satellites, which display increased nucleosome spacing compared with bulk chromatin. While also depleted at active promoters and enriched at major satellites, overexpressed H10 displays differential binding patterns in specific repetitive sequences compared with H1d and H1c. Depletion of H1c, H1d, and H1e causes pericentric chromocenter clustering and de-repression of major satellites. These results integrate the localization of an understudied type of chromatin proteins, namely the H1 variants, into the epigenome map of mouse ESCs, and we identify significant changes at pericentric heterochromatin upon depletion of this epigenetic mark. | en_US |
| dc.identifier.citation | Cao K., Lailler N., Zhang Y., Kumar A., Uppal K., Liu Z., Lee E.K., Wu H., Medrzycki M., Pan C., Ho P.Y., Cooper G.P., Jr, Dong X., Bock C., Bouhassira E.E., Fan Y., "High-resolution mapping of h1 linker histone variants in embryonic stem cells," PLoS Genetics, 9(4):e1003417 (2013). | en_US |
| dc.identifier.doi | 10.1371/journal.pgen.1003417. | |
| dc.identifier.issn | 1553-7390 | |
| dc.identifier.uri | http://hdl.handle.net/1853/49156 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Georgia Institute of Technology | en_US |
| dc.publisher.original | Public Library of Science | en_US |
| dc.subject | Chromatin | en_US |
| dc.subject | Embryonic stem cells | en_US |
| dc.subject | Epigenetics | en_US |
| dc.subject | Gene expression | en_US |
| dc.subject | High performance liquid chromatography | en_US |
| dc.subject | Histones | en_US |
| dc.subject | Mammalian genomics | en_US |
| dc.subject | Nucleosomes | en_US |
| dc.title | High-resolution mapping of h1 linker histone variants in embryonic stem cells. | en_US |
| dc.type | Text | |
| dc.type.genre | Article | |
| dspace.entity.type | Publication | |
| local.contributor.author | Fan, Yuhong | |
| local.contributor.corporatename | College of Sciences | |
| local.contributor.corporatename | School of Biological Sciences | |
| relation.isAuthorOfPublication | 8a6f8119-7916-40bb-8d71-f6c244209e45 | |
| relation.isOrgUnitOfPublication | 85042be6-2d68-4e07-b384-e1f908fae48a | |
| relation.isOrgUnitOfPublication | c8b3bd08-9989-40d3-afe3-e0ad8d5c72b5 |
Files
Original bundle
1 - 1 of 1
- Name:
- Plos_fan_2013_001.pdf
- Size:
- 3.95 MB
- Format:
- Adobe Portable Document Format
- Description: