Title:
The Role of Caveolae in the Loss of ERK2 Activation in Stretched Skeletal Myotubes

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dc.contributor.advisor Burkholder, Thomas
dc.contributor.author Bellott, Anne Claire en_US
dc.contributor.committeeMember Jo, Hanjoong
dc.contributor.committeeMember Merrill, Alfred
dc.contributor.department Mechanical Engineering en_US
dc.date.accessioned 2005-03-02T22:36:21Z
dc.date.available 2005-03-02T22:36:21Z
dc.date.issued 2004-07-12 en_US
dc.description.abstract Skeletal muscle function is important to the human body for daily activities. Mechanical signals are critical to the maintenance of that function. Muscle diseases, such as the muscular dystrophies, in which the force transmission apparatus is compromised, have devastating effects on muscle function and quality of life. Mechanical signals activate intracellular signaling to maintain function. ERK2 has been shown to be quickly and strongly upregulated following stretch, leading to cell proliferation. Stretch has been shown to cause deformation of caveolae, invaginations of the plasma membrane that inhibit ERK signaling. This leads to the hypothesis that stretch induced deformation of caveolae may initiate mechanotransduction by activating ERK2. Reducing caveolin-3 expression via siRNA knockdown eradicated the stretch-induced effect on ERK2 activation, indicating that caveolin is required for the stretch response. Stabilizing caveolae structure by temperature reduction or destabilizing caveolae by cholesterol depletion resulted in changes consistent with the hypothesis that proper caveolae structure plays an important role in inhibition of signaling molecules and that deformation mediates mechanotransduction, resulting in changes in activation of ERK2. en_US
dc.description.degree M.S. en_US
dc.format.extent 1072137 bytes
dc.format.mimetype application/pdf
dc.identifier.uri http://hdl.handle.net/1853/5075
dc.language.iso en_US
dc.publisher Georgia Institute of Technology en_US
dc.subject Skeletal muscle en_US
dc.subject ERK2
dc.subject Mechanotransduction
dc.subject Caveolae
dc.subject Caveolin-3
dc.title The Role of Caveolae in the Loss of ERK2 Activation in Stretched Skeletal Myotubes en_US
dc.type Text
dc.type.genre Thesis
dspace.entity.type Publication
local.contributor.corporatename George W. Woodruff School of Mechanical Engineering
local.contributor.corporatename College of Engineering
relation.isOrgUnitOfPublication c01ff908-c25f-439b-bf10-a074ed886bb7
relation.isOrgUnitOfPublication 7c022d60-21d5-497c-b552-95e489a06569
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