Purification of N-glycosylated E-Cadherin Proteins through Biotinylation Isolation of E-cadherin protein for glycomics study through biotin affinity purification

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PATEL-UNDERGRADUATERESEARCHOPTIONTHESIS-2021.pdf (130.72 KB)
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Patel, Roshni R.
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School of Biological Sciences
School established in 2016 with the merger of the Schools of Applied Physiology and Biology
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Undergraduate Scholarship
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http://hdl.handle.net/1853/66693
Abstract
In this study, we use Xenopus embryos to study the dynamics of neural crest cell migration. This process is important in embryonic development because it initiates the differentiation process of the embryo as a whole. The cell migration is due to the Epithelial to Mesenchymal Transition. For our purposes, we want to study how E-Cadherin changes throughout this migratory process. In particular, the complex sugars (glycans) that are attached to these proteins will be purified and sent for glycomic analysis. My project was not finished due to COVID-19 setbacks. However, we have created a Recombinant DNA construct that has attached an avi-tag to the ends of the E-Cadherin protein sequence. We are currently working on confirming that the construct has been incorporated correctly.
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2021-05
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Undergraduate Thesis
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