Title:
Intracellular drug delivery using laser activated carbon nanoparticles

dc.contributor.advisor Prausnitz, Mark R.
dc.contributor.author Sengupta, Aritra
dc.contributor.committeeMember Thadhani, Naresh N.
dc.contributor.committeeMember Champion, Julie A.
dc.contributor.committeeMember Styczynski, Mark P.
dc.contributor.committeeMember Sambanis, Athanassios
dc.contributor.department Chemical and Biomolecular Engineering
dc.date.accessioned 2015-09-21T15:52:13Z
dc.date.available 2015-09-22T05:30:07Z
dc.date.created 2014-08
dc.date.issued 2014-05-15
dc.date.submitted August 2014
dc.date.updated 2015-09-21T15:52:13Z
dc.description.abstract We demonstrate intracellular delivery of various molecules by inducing controlled and reversible cell damage through pulsed laser irradiation of carbon black (CB) nanoparticles. We then characterized and optimized the system for maximal uptake and minimal loss of viability. At our optimal condition 88% of cells exhibited uptake with almost no loss of viability. In other more intense cases it was shown that cell death could be prevented through addition of poloxamer. The underlying mechanism of action is also studied and our hypothesis is that the laser heats the CB leading to thermal expansion, vapor formation and/or chemical reaction leading to generation of acoustic waves and then there is energy transduction to the cell causing poration of the cell membrane. We also delivered anti-EGFR siRNA to ovarian cancer cells. Cells exposed to a laser at 18.75 mJ/cm2 for 7 minutes resulted in a 49% knockdown of EGFR compared to negative control. We established an alternative way to deliver siRNA to knockdown proteins, for the first time using laser CB interaction.
dc.description.degree Ph.D.
dc.embargo.terms 2015-08-01
dc.format.mimetype application/pdf
dc.identifier.uri http://hdl.handle.net/1853/53996
dc.language.iso en_US
dc.publisher Georgia Institute of Technology
dc.subject Laser
dc.subject Nanosecond
dc.subject Carbon black
dc.subject Intracellular
dc.subject Drug delivery
dc.subject Photoacoustics
dc.subject Pluronics
dc.subject Poloxamer
dc.subject siRNA
dc.subject EGFR
dc.subject In-vivo
dc.subject In-vitro
dc.title Intracellular drug delivery using laser activated carbon nanoparticles
dc.type Text
dc.type.genre Dissertation
dspace.entity.type Publication
local.contributor.advisor Prausnitz, Mark R.
local.contributor.corporatename School of Chemical and Biomolecular Engineering
local.contributor.corporatename College of Engineering
relation.isAdvisorOfPublication 4bd611de-763a-4e92-abdc-0558443485cc
relation.isOrgUnitOfPublication 6cfa2dc6-c5bf-4f6b-99a2-57105d8f7a6f
relation.isOrgUnitOfPublication 7c022d60-21d5-497c-b552-95e489a06569
thesis.degree.level Doctoral
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