Title:
Microfluidics and imaging techniques for high-throughput studies of early embryonic development

dc.contributor.advisor Lu, Hang
dc.contributor.author Levario, Thomas James
dc.contributor.committeeMember Shvartsman, Stanislav Y.
dc.contributor.committeeMember Champion, Julie
dc.contributor.committeeMember Breedveld, Victor
dc.contributor.committeeMember Streelman, Todd
dc.contributor.department Chemical and Biomolecular Engineering
dc.date.accessioned 2017-08-17T18:56:13Z
dc.date.available 2017-08-17T18:56:13Z
dc.date.created 2016-08
dc.date.issued 2016-05-18
dc.date.submitted August 2016
dc.date.updated 2017-08-17T18:56:13Z
dc.description.abstract Understanding how developmental systems achieve robustness is a key goal of developmental biology. The fruit fly Drosophila melanogaster is a model of development and developmental genetics owing to high genetic conservation that can provide insight into human development. Drosophila is compatible with in vivo live imaging: a powerful technique that allows researchers to visualize dynamic processes in real time within developing organisms, but is technically challenging to perform. As a result, large-scale data collection is virtually impossible preventing researchers from obtaining highly quantitative information regarding live embryo development. To address this issue, this thesis advances the quantitative imaging toolsets available to biologists by developing microfluidic technologies for high-throughput time-lapse microscopy of live Drosophila embryos as well as image processing and analysis software for automated quantitative phenotyping of dynamic processes. Significant engineering feats allowed for the expansion of microsystem functionality and integration with computer vision algorithms facilitate rapid microscopy and quantitative analysis of a wide range of biological applications. As a result of these technological advances, insight regarding anoxia-induced developmental arrest and recovery, mitotic wave-front propagation dynamics, and the effects of RTK-ERK pathway mutations on downstream signaling kinetics were uncovered and quantitatively characterized. The technologies developed in this dissertation are generalizable, and should facilitate rapid microscopy and quantitative phenotyping throughout developmental biology.
dc.description.degree Ph.D.
dc.format.mimetype application/pdf
dc.identifier.uri http://hdl.handle.net/1853/58573
dc.language.iso en_US
dc.publisher Georgia Institute of Technology
dc.subject Microfluidics
dc.subject Developmental biology
dc.title Microfluidics and imaging techniques for high-throughput studies of early embryonic development
dc.type Text
dc.type.genre Dissertation
dspace.entity.type Publication
local.contributor.advisor Lu, Hang
local.contributor.corporatename School of Chemical and Biomolecular Engineering
local.contributor.corporatename College of Engineering
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relation.isOrgUnitOfPublication 6cfa2dc6-c5bf-4f6b-99a2-57105d8f7a6f
relation.isOrgUnitOfPublication 7c022d60-21d5-497c-b552-95e489a06569
thesis.degree.level Doctoral
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