Title:
Substrate-level control of glucose metabolism in C2C12 myotubes

dc.contributor.advisor Burkholder, Thomas
dc.contributor.author Hsu, Chia
dc.contributor.committeeMember Millard-Stafford, Mindy
dc.contributor.committeeMember Balog, Edward
dc.contributor.committeeMember Jang, Young
dc.contributor.committeeMember Price, S. Russ
dc.contributor.corporatename School of Applied Physiology
dc.contributor.corporatename School of Biological Sciences
dc.contributor.corporatename College of Sciences
dc.contributor.corporatename College of Sciences
dc.contributor.corporatename School of Biological Sciences
dc.contributor.department Applied Physiology
dc.date.accessioned 2017-06-07T17:37:48Z
dc.date.available 2017-06-07T17:37:48Z
dc.date.created 2016-05
dc.date.issued 2016-03-30
dc.date.submitted May 2016
dc.date.updated 2017-06-07T17:37:48Z
dc.description.abstract Metabolic flexibility is critical for muscle to maintain proper function and overall health. Muscle adapts to metabolic stress with increasing ATP synthesis by enhancing the rate of glycolysis and mitochondrial respiration. The control of that rate is mediated by several glucose metabolites. This project is based on the conceptual model that AMP indicates the balance of ATP synthesis and degradation, and NADH indicates the balance of glucose delivery to oxygen delivery. AMP signaling facilitates all aspects of glucose metabolism, and NAD+ signaling facilitates oxidative metabolism and inhibits reductive metabolism. The overall hypothesis is that the distribution of glucose depends on AMP and NAD+ generated during energetic stress. The results suggest that glucose metabolism is highly sensitive to ATP homeostasis via AMPK activity. NADH oxidation alone is not sufficient to influence glucose oxidation, but require co-activation of AMPK. AMP and NAD+ signaling work independently in metabolic gene expression. The overall conclusion is that glucose metabolism depends on AMP signaling, but NAD signaling is unable to alter glucose disposal. AMP and NAD independently induced metabolic and differentiation adaptation. These findings suggest that other molecule may represent an additional gauge of aerobic and anaerobic metabolism.
dc.description.degree Ph.D.
dc.format.mimetype application/pdf
dc.identifier.uri http://hdl.handle.net/1853/58176
dc.language.iso en_US
dc.publisher Georgia Institute of Technology
dc.subject AMP
dc.subject AMPK
dc.subject NAD
dc.subject NADH
dc.subject p38
dc.subject Glucose metabolism
dc.subject SIRT1
dc.title Substrate-level control of glucose metabolism in C2C12 myotubes
dc.type Text
dc.type.genre Dissertation
dspace.entity.type Publication
local.contributor.corporatename College of Sciences
local.contributor.corporatename School of Biological Sciences
local.relation.ispartofseries Doctor of Philosophy with a Major in Applied Physiology
relation.isOrgUnitOfPublication 85042be6-2d68-4e07-b384-e1f908fae48a
relation.isOrgUnitOfPublication c8b3bd08-9989-40d3-afe3-e0ad8d5c72b5
relation.isOrgUnitOfPublication 85042be6-2d68-4e07-b384-e1f908fae48a
relation.isSeriesOfPublication ead85f7a-56bd-4216-a2d8-a66530e2e8b9
thesis.degree.level Doctoral
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