Title:
Long-Range Charge Transfer in Plasmid DNA Condensates and DNA-Directed Assembly of Conducting Polymers

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dc.contributor.advisor Schuster, Gary B.
dc.contributor.author Das, Prolay en_US
dc.contributor.committeeMember Collard, David M.
dc.contributor.committeeMember Donald Doyle
dc.contributor.committeeMember Weck, Marcus
dc.contributor.committeeMember Uzi Landman
dc.contributor.department Chemistry and Biochemistry en_US
dc.date.accessioned 2008-02-07T18:49:26Z
dc.date.available 2008-02-07T18:49:26Z
dc.date.issued 2007-11-12 en_US
dc.description.abstract Long-distance radical cation transport was studied in DNA condensates where linearized pUC19 plasmid was ligated to an oligomer and transformed into DNA condensates with spermidine. DNA condensates were detected by Dynamic Light Scattering and observed by Transmission Electron Microscopy. Introduction of charge into the condensates causes long-distance charge migration, which is detected by reaction at the remote guanines. The efficiency of charge migration in the condensate is significantly less than it is for the corresponding oligomer in solution. This result is attributed to a lower mobility for the migrating radical cation in the condensate, caused by inhibited formation of charge-transfer-effective states. Radical cation transport was also studied in DNA condensates made from an oligomer sandwiched between two linearized plasmids by double ligation. Unlike the single ligated plasmid condensates, the efficiency of charge migration in the double ligated plasmid-condensates is high, indicative of local structural and conformational transformation of the DNA duplexes. Organic monomer units having extended ð-conjugation as part of a long conducting polymer was synthesized and characterized. The monomer units were covalently attached to particular positions in DNA oligonucleotides by either the convertible nucleotide approach or by phosphoramidite chemistry. Successful attachment of the monomer units to DNA were confirmed by mass spectral analysis. The DNA-conjoined monomer units can self assemble in the presence of complementary sequences which act as templates that can control polymer formation and structure. By this method the para-direction of the polymer formation can be enforced and may be used to generate materials having nonrecurring, irregular structures. en_US
dc.description.degree Ph.D. en_US
dc.identifier.uri http://hdl.handle.net/1853/19856
dc.publisher Georgia Institute of Technology en_US
dc.subject DNA charge transfer en_US
dc.subject Conducting polymers en_US
dc.subject Radical cation en_US
dc.subject Self assembly en_US
dc.subject.lcsh Conducting polymers
dc.subject.lcsh Charge tranfer in biology
dc.subject.lcsh Cations
dc.subject.lcsh DNA
dc.subject.lcsh Self-organizing systems
dc.title Long-Range Charge Transfer in Plasmid DNA Condensates and DNA-Directed Assembly of Conducting Polymers en_US
dc.type Text
dc.type.genre Dissertation
dspace.entity.type Publication
local.contributor.advisor Schuster, Gary B.
local.contributor.corporatename School of Chemistry and Biochemistry
local.contributor.corporatename College of Sciences
relation.isAdvisorOfPublication 5496b90a-0188-49d8-9ec7-fa1ee916e66e
relation.isOrgUnitOfPublication f1725b93-3ab8-4c47-a4c3-3596c03d6f1e
relation.isOrgUnitOfPublication 85042be6-2d68-4e07-b384-e1f908fae48a
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