Molecular tools for elucidating copper biochemistry: Water-soluble fluorescent probes and robust affinity standards

Morgan, M. Thomas

Title:

Molecular tools for elucidating copper biochemistry: Water-soluble fluorescent probes and robust affinity standards

dc.contributor.advisor	Fahrni, Christoph J.
dc.contributor.author	Morgan, M. Thomas
dc.contributor.committeeMember	Perry, Joseph W.
dc.contributor.committeeMember	Taite, Lakeshia J.
dc.contributor.committeeMember	Tolbert, Laren M.
dc.contributor.committeeMember	Marder, Seth R.
dc.contributor.department	Chemistry and Biochemistry
dc.date.accessioned	2014-05-28T23:23:50Z
dc.date.available	2014-05-28T23:23:50Z
dc.date.issued	2013-04-09
dc.description.abstract	Copper is an essential trace element for living organisms and has both known and additional suspected roles in human health and disease. The current understanding of copper metabolism is substantial but incomplete, particularly in regard to storage and exchange at the subcellular level, although available evidence indicates exchangeable intracellular copper is in the monovalent oxidation state. Selective fluorescent probes with sufficient sensitivity to detect Cu(I) availability at physiologically relevant levels and at subcellular resolution would be valuable tools for studying copper metabolism. As a contribution toward this goal, this work describes the development of Cu(I)-selective fluorescent probes with greatly improved aqueous solubility, contrast ratio, and fluorescence quantum yield. This work also describes the development of water-soluble, 1:1-binding chelators that form colorless, air-stable copper(I)-complexes. By acting as copper(I) buffering agents and affinity standards, these compounds can serve a complementary role to fluorescent probes in the study of copper biochemistry.	en_US
dc.description.degree	Ph.D.
dc.embargo.terms	null	en_US
dc.identifier.uri	http://hdl.handle.net/1853/51937
dc.language.iso	en_US	en_US
dc.publisher	Georgia Institute of Technology	en_US
dc.subject	CTAP-2	en_US
dc.subject	Sulfonyl fluoride	en_US
dc.subject	Sulfonate	en_US
dc.subject	Neopentyl	en_US
dc.subject	Triarylpyrazoline	en_US
dc.subject	Copper(I)	en_US
dc.subject	Photoinduced electron transfer	en_US
dc.subject	Fluorophore	en_US
dc.subject	ESPT	en_US
dc.subject	Ligand	en_US
dc.subject	Aqueous	en_US
dc.subject.lcsh	Copper in the body
dc.subject.lcsh	Biochemistry
dc.subject.lcsh	Fluorescent probes
dc.title	Molecular tools for elucidating copper biochemistry: Water-soluble fluorescent probes and robust affinity standards	en_US
dc.type	Text
dc.type.genre	Dissertation
dspace.entity.type	Publication
local.contributor.advisor	Fahrni, Christoph J.
local.contributor.corporatename	School of Chemistry and Biochemistry
local.contributor.corporatename	College of Sciences
relation.isAdvisorOfPublication	582e68ff-4689-4d69-95bd-97bbaf927034
relation.isOrgUnitOfPublication	f1725b93-3ab8-4c47-a4c3-3596c03d6f1e
relation.isOrgUnitOfPublication	85042be6-2d68-4e07-b384-e1f908fae48a
thesis.degree.level	Doctoral