Title:
Developing Ion Mobility-Mass Spectrometry Techniques to Increase Sensitivity and Resolution for Carbohydrate Mixture Analysis
Developing Ion Mobility-Mass Spectrometry Techniques to Increase Sensitivity and Resolution for Carbohydrate Mixture Analysis
| dc.contributor.advisor | Fernández, Facundo M. | |
| dc.contributor.author | Mckenna, Kristin Ruth | |
| dc.contributor.committeeMember | Wu, Ronghu | |
| dc.contributor.committeeMember | Stockton, Amanda | |
| dc.contributor.committeeMember | Liotta, Charles L. | |
| dc.contributor.committeeMember | Krishnamurthy, Ramanarayanan | |
| dc.contributor.department | Chemistry and Biochemistry | |
| dc.date.accessioned | 2022-01-14T16:06:43Z | |
| dc.date.available | 2022-01-14T16:06:43Z | |
| dc.date.created | 2021-12 | |
| dc.date.issued | 2021-08-19 | |
| dc.date.submitted | December 2021 | |
| dc.date.updated | 2022-01-14T16:06:43Z | |
| dc.description.abstract | The origin and prebiotic functions of carbohydrates are not well characterized. Limitations in analytical methodology to analyze the regio- and stereochemistry of carbohydrates in complex mixtures exacerbates this problem. Several ion mobility-tandem mass spectrometry techniques were developed to study model prebiotic carbohydrate reactions. Covalent derivatization with 3-carboxy-5-nitrophenylboronic acid (3C5NBA) was determined to improve these characterizations and allow for more complete structural analysis by tandem mass spectrometry. Cyclic ion mobility spectrometry improved the ability to distinguish four monosaccharide and eight disaccharide isomers as their 3C5NBA derivatives. Organic acids were also analyzed for their potential to improve carbohydrate separations as noncovalent modifiers. The optimal organic acid modifiers were determined to be L-malic acid and N-methyl-D-aspartic acid, which were further characterized through a more sensitive, Fourier transform-based ion mobility method. | |
| dc.description.degree | Ph.D. | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.uri | http://hdl.handle.net/1853/66053 | |
| dc.language.iso | en_US | |
| dc.publisher | Georgia Institute of Technology | |
| dc.subject | Ion mobility spectrometry | |
| dc.subject | Mass spectrometry | |
| dc.subject | Tandem mass spectrometry | |
| dc.subject | Carbohydrates | |
| dc.subject | Origins of life chemistry | |
| dc.title | Developing Ion Mobility-Mass Spectrometry Techniques to Increase Sensitivity and Resolution for Carbohydrate Mixture Analysis | |
| dc.type | Text | |
| dc.type.genre | Dissertation | |
| dspace.entity.type | Publication | |
| local.contributor.advisor | Fernández, Facundo M. | |
| local.contributor.corporatename | School of Chemistry and Biochemistry | |
| local.contributor.corporatename | College of Sciences | |
| relation.isAdvisorOfPublication | 60fbfa01-4d0f-42eb-93b1-7577bf996e40 | |
| relation.isOrgUnitOfPublication | f1725b93-3ab8-4c47-a4c3-3596c03d6f1e | |
| relation.isOrgUnitOfPublication | 85042be6-2d68-4e07-b384-e1f908fae48a | |
| thesis.degree.level | Doctoral |