Title:
Rapid Multiplexing of Proteins for Deciphering Spatially Resolved Biophysical Organelle Networks

dc.contributor.advisor Coskun, Ahmet F.
dc.contributor.author Yajima, Yukina
dc.contributor.committeeMember Venkatesan, Mythreye
dc.contributor.department Biomedical Engineering (Joint GT/Emory Department)
dc.date.accessioned 2022-05-27T14:37:41Z
dc.date.available 2022-05-27T14:37:41Z
dc.date.created 2022-05
dc.date.issued 2022-05
dc.date.submitted May 2022
dc.date.updated 2022-05-27T14:37:41Z
dc.description.abstract Organelles play important roles in human health and disease, such as maintaining homeostasis, regulating growth and aging, and generating energy. Organelle diversity in cells not only exists between cell types but also between individual cells. Therefore, studying the morphological features of organelles at the single-cell level is important to understand the functioning of cells. Mesenchymal stem cells are multipotent cells that have been explored as a therapeutic for treating a variety of diseases, including myocardial infarction and traumatic brain injury. Studying how organelles are structured in these cells can answer questions about their function and potential. After a rapid multiplexed immunofluorescence was performed to understand the spatial organization of 10 organelle proteins, a morphological analysis was performed for features such as the area, intensity, and perimeter. The analysis was performed on 14 selected cells, 7 taken from umbilical cord and 7 taken from bone marrow. This approach aims to explore relations between the organelles and compare the two cell types, which can aid in better understanding various microenvironments and to develop personalized stem cell therapeutics.
dc.description.degree Undergraduate
dc.format.mimetype application/pdf
dc.identifier.uri http://hdl.handle.net/1853/66717
dc.language.iso en_US
dc.publisher Georgia Institute of Technology
dc.subject Stem cells
dc.subject Organelles
dc.subject Imaging
dc.subject Immunofluorescence
dc.subject Image analysis
dc.title Rapid Multiplexing of Proteins for Deciphering Spatially Resolved Biophysical Organelle Networks
dc.type Text
dc.type.genre Undergraduate Thesis
dspace.entity.type Publication
local.contributor.corporatename Wallace H. Coulter Department of Biomedical Engineering
local.contributor.corporatename Undergraduate Research Opportunities Program
local.contributor.corporatename College of Engineering
local.relation.ispartofseries Undergraduate Research Option Theses
relation.isOrgUnitOfPublication da59be3c-3d0a-41da-91b9-ebe2ecc83b66
relation.isOrgUnitOfPublication 0db885f5-939b-4de1-807b-f2ec73714200
relation.isOrgUnitOfPublication 7c022d60-21d5-497c-b552-95e489a06569
relation.isSeriesOfPublication e1a827bd-cf25-4b83-ba24-70848b7036ac
thesis.degree.level Undergraduate
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
YAJIMA-UNDERGRADUATERESEARCHOPTIONTHESIS-2022.pdf
Size:
2.63 MB
Format:
Adobe Portable Document Format
Description:
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
LICENSE.txt
Size:
3.87 KB
Format:
Plain Text
Description: