Title:
BIOPRINTED CARDIAC PATCH COMPOSED OF CARDIAC PROGENITOR CELLS AND EXTRACELLULAR MATRIX FOR HEART REPAIR AND REGENERATION

dc.contributor.advisor Davis, Michael E.
dc.contributor.author Bejleri, Donald
dc.contributor.committeeMember Temenoff, Johnna S.
dc.contributor.committeeMember Platt, Manu O.
dc.contributor.committeeMember Cho, Hee Cheol
dc.contributor.committeeMember Champion, Julie A.
dc.contributor.department Biomedical Engineering (Joint GT/Emory Department)
dc.date.accessioned 2022-01-14T16:02:25Z
dc.date.available 2022-01-14T16:02:25Z
dc.date.created 2020-12
dc.date.issued 2020-08-24
dc.date.submitted December 2020
dc.date.updated 2022-01-14T16:02:25Z
dc.description.abstract Congenital heart defects are present in 8 of 1000 newborns, and palliative surgical therapy has increased survival rates. Despite improved outcomes, many children develop reduced cardiac function and go on to heart failure and transplantation. Human cardiac progenitor cell (hCPC) therapy has the potential to repair the pediatric myocardium through reparative factor release but suffers from limited hCPC retention and functionality. Decellularized cardiac extracellular matrix hydrogel (cECM) has improved heart function in adults while also improving CPC functionality in 2D and 3D culture. This work focuses on developing a bioprinted cardiac patch composed of native cECM and pediatric hCPCs, for use as an epicardial device in repairing the damaged myocardium. First, a method to print patches with bioinks composed of cECM, hCPCs, and gelatin methacrylate (GelMA) is developed. Patch assessments include bioink printability, cellular functionality, and mechanical properties in vitro. To further tailor the reparative potential of cardiac patches, modifying patch components, particularly cell age, matrix composition, and oxygen growth conditions are evaluated. Finally, the implantation of patches in vivo towards improvements to cardiac function in a rat model of right ventricular heart failure, compared to sham controls and cell-free patches, is evaluated. Assessments include hCPC retention, right ventricle function, and tissue level parameters (vessel density, cardiomyocyte hypertrophy, and fibrosis) across all treatments. The animal model evaluation shows that cell-free and neonatal hCPC-laden cECM-GelMA patches improve right ventricle function and tissue level parameters compared to other patch groups and surgical controls. cECM inclusion into patches may be the most influential parameter driving therapeutic improvements. Additionally, child hCPC patches require cECM incorporation to improve right ventricle function, compared to cECM-free child hCPC patches. Altogether, this study paves the way for clinical trials in treating pediatric heart failure using the bioprinted hCPC-GelMA-cECM patches.
dc.description.degree Ph.D.
dc.format.mimetype application/pdf
dc.identifier.uri http://hdl.handle.net/1853/65978
dc.language.iso en_US
dc.publisher Georgia Institute of Technology
dc.subject Bioprinting
dc.subject cardiac regeneration
dc.subject cardiac extracellular matrix
dc.subject cardiac progenitor cells
dc.title BIOPRINTED CARDIAC PATCH COMPOSED OF CARDIAC PROGENITOR CELLS AND EXTRACELLULAR MATRIX FOR HEART REPAIR AND REGENERATION
dc.type Text
dc.type.genre Dissertation
dspace.entity.type Publication
local.contributor.advisor Davis, Michael E.
local.contributor.corporatename Wallace H. Coulter Department of Biomedical Engineering
local.contributor.corporatename College of Engineering
relation.isAdvisorOfPublication fab83195-e1b0-4b5e-933d-5b97a14b945a
relation.isOrgUnitOfPublication da59be3c-3d0a-41da-91b9-ebe2ecc83b66
relation.isOrgUnitOfPublication 7c022d60-21d5-497c-b552-95e489a06569
thesis.degree.level Doctoral
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
BEJLERI-DISSERTATION-2020.pdf
Size:
4.87 MB
Format:
Adobe Portable Document Format
Description:
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
LICENSE.txt
Size:
3.87 KB
Format:
Plain Text
Description: