Visualizing Biological Machines with CryoEM

Carragher, Bridget

Title:

Visualizing Biological Machines with CryoEM

dc.contributor.author	Carragher, Bridget
dc.contributor.corporatename	Georgia Institute of Technology. School of Biology
dc.contributor.corporatename	Scripps Research Institute
dc.date.accessioned	2008-10-28T14:14:56Z
dc.date.available	2008-10-28T14:14:56Z
dc.date.issued	2008-09-22
dc.description	Presented on September 22, 2008 from 11:00 AM – 12:00 PM in room 1128 of the Parker H. Petit Institute for Bioengineering and Bioscience on the Georgia Tech campus	en
dc.description	Runtime: 53:17 minutes
dc.description.abstract	Over the past decade, cryo-electron microscopy (cryoEM) has emerged as a powerful approach to the structural determination of large macromolecular complexes. Elucidating the structure and mechanism of action of these "molecular machines" is an emerging frontier in understanding how the information in the genome is transformed into cellular activities. In cryoEM the macro molecular specimen is preserved in a thin layer of vitreous (glassy) ice and imaged in the electron microscope using very low doses of electrons. The low signal to noise ratio of the resulting images means that averaging is required to recover the signal and reconstruct a three dimensional map of the structure. Our goal is to develop a pipeline to automate the processes involved in solving macromolecular structures using cryo-electron microscopy. One of the goals of the pipeline is to enable much higher data throughputs and improve the resolution of single particle reconstructions. We are also using the pipeline to help understand what currently limits resolution in these maps. The current status of these efforts will be illustrated using a variety of macromolecules as case studies.	en
dc.format.extent	53:17 minutes
dc.identifier.uri	http://hdl.handle.net/1853/25357
dc.language.iso	en_US	en
dc.publisher	Georgia Institute of Technology	en
dc.subject	CryoEM	en
dc.subject	Cryo-electron microscopy	en
dc.subject	Automation	en
dc.subject	Molecular machines	en
dc.title	Visualizing Biological Machines with CryoEM	en
dc.type	Moving Image
dc.type	Text
dc.type.genre	Lecture
dc.type.genre	Presentation
dspace.entity.type	Publication
local.relation.ispartofseries	School of Biology Seminar Series
relation.isSeriesOfPublication	146d0d36-ad75-4642-ad2a-96dcbf372f06