Title:
Oscillatory Compressive Loading Effects On Mesenchymal Progenitor Cells Undergoing Chondrogenic Differentiation In Hydrogel Suspension

Simple item page
dc.contributor.advisor Guldberg, Robert E.
dc.contributor.author Case, Natasha D. en_US
dc.contributor.committeeMember García, Andrés J.
dc.contributor.committeeMember Anthony Ratcliffe
dc.contributor.committeeMember Boyan, Barbara D.
dc.contributor.committeeMember Levenston, Marc E.
dc.contributor.department Biomedical Engineering en_US
dc.date.accessioned 2005-07-28T18:03:27Z
dc.date.available 2005-07-28T18:03:27Z
dc.date.issued 2005-04-15 en_US
dc.description.abstract Articular cartilage functions to maintain joint mobility. The loss of healthy, functional articular cartilage due to osteoarthritis or injury can severely compromise quality of life. To address this issue, cartilage tissue engineering approaches are currently in development. Bone marrow-derived mesenchymal progenitor cells (MPCs) hold much promise as an alternative cell source for cartilage tissue engineering. While previous studies have established that MPCs from humans and multiple other species undergo in vitro chondrogenic differentiation, additional research is needed to define conditions that will enhance MPC differentiation, increase matrix production by differentiating cultures, and support development of functional tissue-engineered cartilage constructs. Mechanical loading may be an important factor regulating chondrogenic differentiation of MPCs and cartilage matrix formation by chondrogenic MPCs. This thesis work evaluated the influence of oscillatory unconfined compressive mechanical loading on in vitro MPC chondrogenic activity and biosynthesis within hydrogel suspension. Loading was conducted using MPCs cultured in media supplements supporting chondrogenic differentiation. Possible interactions between the number of days in chondrogenic media preceding loading initiation and the ability of the MPC culture to respond to mechanical stimulation were explored in two different loading studies. The first loading study investigated the effects of 3 hour periods of daily oscillatory mechanical stimulation on subsequent chondrogenic activity, where chondrogenic activity represented an assessment of cartilage matrix production by differentiating MPCs. This study found that oscillatory compression of MPCs initiated during the first seven days of culture did not enhance chondrogenic activity above the level supported by media supplements alone. The second loading study evaluated changes in biosynthesis during a single 20 hour period of oscillatory mechanical stimulation to assess mechanoresponsiveness of the MPC cultures. This study found that MPCs modulated proteoglycan and protein synthesis in a culture time-dependent and frequency-dependent manner upon application of oscillatory compression. Together the two loading studies provide an assessment of dynamic compressive mechanical loading influences on MPC cultures undergoing chondrogenic differentiation. The information gained through in vitro studies of differentiating MPC cultures will increase basic knowledge about progenitor cells and may also prove valuable in guiding the future development of cartilage tissue engineering approaches. en_US
dc.description.degree Ph.D. en_US
dc.format.extent 1713130 bytes
dc.format.mimetype application/pdf
dc.identifier.uri http://hdl.handle.net/1853/6939
dc.language.iso en_US
dc.publisher Georgia Institute of Technology en_US
dc.subject Compressive loading en_US
dc.subject Mechanical stimulation
dc.subject Chondrogenic differentiation
dc.subject Mesenchymal progenitor cell
dc.subject Alginate
dc.subject TGF-beta 1
dc.title Oscillatory Compressive Loading Effects On Mesenchymal Progenitor Cells Undergoing Chondrogenic Differentiation In Hydrogel Suspension en_US
dc.type Text
dc.type.genre Dissertation
dspace.entity.type Publication
local.contributor.advisor Guldberg, Robert E.
local.contributor.corporatename Wallace H. Coulter Department of Biomedical Engineering
local.contributor.corporatename College of Engineering
relation.isAdvisorOfPublication 5de086eb-63e8-46e3-b1cc-3569bb13e59c
relation.isOrgUnitOfPublication da59be3c-3d0a-41da-91b9-ebe2ecc83b66
relation.isOrgUnitOfPublication 7c022d60-21d5-497c-b552-95e489a06569
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
case_natasha_d_200505_phd.pdf
Size:
1.63 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Theses and Dissertations