Gene expression profiling of epithelial ovarian tumours correlated with malignant potential

Warrenfeltz, Susanne; Pavlik, Stephen; Datta, Susmita; Kraemer, Eileen T.; Benigno, Benedict B.; McDonald, John F.

Title:

Gene expression profiling of epithelial ovarian tumours correlated with malignant potential

dc.contributor.author	Warrenfeltz, Susanne	en_US
dc.contributor.author	Pavlik, Stephen	en_US
dc.contributor.author	Datta, Susmita	en_US
dc.contributor.author	Kraemer, Eileen T.	en_US
dc.contributor.author	Benigno, Benedict B.	en_US
dc.contributor.author	McDonald, John F.	en_US
dc.contributor.corporatename	University of Georgia. Dept. of Genetics	en_US
dc.contributor.corporatename	Georgia Institute of Technology. Ovarian Cancer Institute	en_US
dc.contributor.corporatename	University of Georgia. Dept. of Computer Science	en_US
dc.contributor.corporatename	Georgia State University. Dept. of Mathematics and Statistics	en_US
dc.date.accessioned	2010-03-23T15:42:10Z
dc.date.available	2010-03-23T15:42:10Z
dc.date.issued	2004-10-07
dc.description	© 2004 Warrenfeltz et al; licensee BioMed Central Ltd.The electronic version of this article is the complete one and can be found online at: http://www.molecular-cancer.com/content/3/1/27	en
dc.description	DOI: 10.1186/1476-4598-3-27
dc.description.abstract	Background Epithelial ovarian tumours exhibit a range of malignant potential, presenting distinct clinical phenotypes. Improved knowledge of gene expression changes and functional pathways associated with these clinical phenotypes may lead to new treatment targets, markers for early detection and a better understanding of disease progression. Results Gene expression profiling (Affymetrix, U95Av2) was carried out on 18 ovarian tumours including benign adenomas, borderline adenocarcinomas of low malignant potential and malignant adenocarcinomas. Clustering the expression profiles of samples from patients not treated with chemotherapy prior to surgery effectively classified 92% of samples into their proper histopathological group. Some cancer samples from patients treated with chemotherapy prior to surgery clustered with the benign adenomas. Chemotherapy patients whose tumours exhibited benign-like expression patterns remained disease free for the duration of this study as indicated by continued normal serum CA-125 levels. Statistical analysis identified 163 differentially expressed genes: 61 genes under-expressed in cancer and 102 genes over-expressed in cancer. Profiling the functional categories of co-ordinately expressed genes within this list revealed significant correlation between increased malignant potential and loss of both IGF binding proteins and cell adhesion molecules. Interestingly, in several instances co-ordinately expressed genes sharing biological function also shared chromosomal location. Conclusion Our findings indicate that gene expression profiling can reliably distinguish between benign and malignant ovarian tumours. Expression profiles of samples from patients pre-treated with chemotherapy may be useful in predicting disease free survival and the likelihood of recurrence. Loss of expression of IGF binding proteins as well as specific cell adhesion molecules may be a significant mechanism of disease progression in ovarian cancer. Expression levels in borderline tumours were intermediate between benign adenomas and malignant adenocarcinomas for a significant portion of the differentially expressed genes, suggesting that borderline tumours are a transitional state between benign and malignant tumours. Finally, genes displaying coordinated changes in gene expression were often genetically linked, suggesting that changes in expression for these genes are the consequence of regional duplications, deletions or epigenetic events.	en
dc.identifier.citation	Susanne Warrenfeltz, Stephen Pavlik, Susmita Datta, Eileen T. Kraemer, Benedict Benigno and John F. McDonald, "Gene expression profiling of epithelial ovarian tumours correlated with malignant potential," Molecular Cancer 2004, 3:27	en
dc.identifier.doi	10.1186/1476-4598-3-27
dc.identifier.issn	1476-4598
dc.identifier.uri	http://hdl.handle.net/1853/32466
dc.language.iso	en_US	en
dc.publisher	Georgia Institute of Technology	en
dc.publisher.original	BioMed Central
dc.subject	Ovarian tumors	en
dc.subject	Gene expression profiling	en
dc.subject	Histopathological groups	en
dc.subject	Recurrence	en
dc.subject	Malignant potential	en
dc.title	Gene expression profiling of epithelial ovarian tumours correlated with malignant potential	en
dc.type	Text
dc.type.genre	Article
dspace.entity.type	Publication
local.contributor.author	McDonald, John F.
local.contributor.corporatename	College of Sciences
local.contributor.corporatename	School of Biological Sciences
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