Title:
Effect of Netropsin on One-electron Oxidation of DNA

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dc.contributor.advisor Schuster, Gary B.
dc.contributor.author Roberts, Lezah Wilette en_US
dc.contributor.committeeMember Donald Doyle
dc.contributor.committeeMember Laren Tolbert
dc.contributor.committeeMember Hud, Nicholas V.
dc.contributor.committeeMember Roger Wartell
dc.contributor.department Chemistry and Biochemistry en_US
dc.date.accessioned 2005-09-16T15:15:52Z
dc.date.available 2005-09-16T15:15:52Z
dc.date.issued 2005-07-19 en_US
dc.description.abstract One electron oxidation of DNA has been studied extensively over the years. When a charge is injected into a DNA duplex, it migrates through the DNA until it reaches a trap. Upon further reactions, damage occurs in this area and strand cleavage can occur. Many works have been performed to see what can affect this damage to DNA. Netropsin is a minor groove binder that can bind to tracts of four to five A:T base pairs. It has been used in the studies within to determine if it can protect DNA against oxidative damage, caused by one-electron oxidation, when it is bound within the minor groove of the DNA. By using a naphthacenedione derivative as a photosensitizer, several DNA duplexes containing netropsin binding sites as well as those without binding sites, were irradiated at 420 nm, analyzed, and visualized to determine its effect on oxidative damage. It has been determined netropsin creates a quenching sphere of an average of 5.8 * 108 Šwhether bound to the DNA or not. Herein we will show netropsin protects DNA against oxidative damage whether it is free in solutions or bound within the minor groove of a DNA duplex. en_US
dc.description.degree Ph.D. en_US
dc.format.extent 2606225 bytes
dc.format.mimetype application/pdf
dc.identifier.uri http://hdl.handle.net/1853/7228
dc.language.iso en_US
dc.publisher Georgia Institute of Technology en_US
dc.subject Photosensitizer en_US
dc.subject Charge transfer
dc.subject Netropsin
dc.subject TQ
dc.subject DNA
dc.subject Electron transfer
dc.subject One-electron oxidation
dc.subject.lcsh Photosensitization, Biological en_US
dc.subject.lcsh Charge transfer in biology en_US
dc.subject.lcsh DNA Analysis en_US
dc.subject.lcsh Oxidation, Physiological en_US
dc.title Effect of Netropsin on One-electron Oxidation of DNA en_US
dc.type Text
dc.type.genre Dissertation
dspace.entity.type Publication
local.contributor.advisor Schuster, Gary B.
local.contributor.corporatename School of Chemistry and Biochemistry
local.contributor.corporatename College of Sciences
relation.isAdvisorOfPublication 5496b90a-0188-49d8-9ec7-fa1ee916e66e
relation.isOrgUnitOfPublication f1725b93-3ab8-4c47-a4c3-3596c03d6f1e
relation.isOrgUnitOfPublication 85042be6-2d68-4e07-b384-e1f908fae48a
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