Biochemical and Biophysical Characterization of Archaeal Intramembrane Aspartyl Proteases – Decoding Substrate Specificity, Kinetic Properties, and Solution Structure
Author(s)
Wu, Yuqi
Advisor(s)
Editor(s)
Collections
Supplementary to:
Permanent Link
Abstract
Intramembrane proteolysis is a conserved biochemical process, where the cleavage products are involved in diverse critical signaling events including cell differentiation, development, immune response and surveillance, and cholesterol metabolism. Conversely, IP dysfunction is often associated with human diseases. However, a complicated ternary complex composed of intramembrane protease (IP), transmembrane substrate, and lipid environment is always present and remains a major challenge in the study of IPs. Intramembrane aspartyl proteases, or IAPs, are least understood despite an increasing number of solved structures in recent years. In this work, we aim to understand IAP substrate specificity, kinetic properties, and solution structure through various biochemical and biophysical techniques and how lipid environment plays a role.
Sponsor
Date
2023-08-02
Extent
Resource Type
Text
Resource Subtype
Dissertation