Title:
Development and Utilization of Genetic Probes for Studying Zebra Mussel Veligers
Development and Utilization of Genetic Probes for Studying Zebra Mussel Veligers
Author(s)
Frischer, Marc E.
Wyllie, Jane A.
Hansen, Andrew S.
Nierzwicki-Bauer, Sandra A.
Wyllie, Jane A.
Hansen, Andrew S.
Nierzwicki-Bauer, Sandra A.
Advisor(s)
Editor(s)
Hatcher, Kathryn J.
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Abstract
A key issue in the management of zebra mussel
populations is early, rapid, and accurate detection of the
planktonic forms of the mussel veligers. In this study, an
alternative technology to microscopic enumeration of zebra
mussel veligers has been developed based on a species specific
genetic probe targeting the 18S rRNA molecule. Probe
specificity and sensitivity were determined empirically in the
laboratory. The probe did not hybridize to any other bivalve
representative tested, including a close relative of the zebra
mussel, Mytilopsis leucophaeta. A single veliger could be
detected by 18S rRNA targeted probes. The probe was tested in
three different field settings including the Hudson River, NY,
Lake Champlain, NY/VT, and Lake George, NY and compared
to standard methods. Results were generally consistent with
microscopy methods. A long term goal of this work is the
application of genetic probe technology to the development of a
field device for the routine detection and quantification of zebra
mussel veligers.
Sponsor
Sponsored and Organized by: U.S. Geological Survey, Georgia Department of Natural Resources, The University of Georgia, Georgia State University, Georgia Institute of Technology
Date Issued
1997-03
Extent
Resource Type
Text
Resource Subtype
Proceedings