Title:
FRAMEWORK FOR A FIBRIN-SPECIFIC APTAMER FOR TARGETED DELIVERY OF FIBRINOLYTIC ESCHERICHIA COLI
FRAMEWORK FOR A FIBRIN-SPECIFIC APTAMER FOR TARGETED DELIVERY OF FIBRINOLYTIC ESCHERICHIA COLI
Author(s)
White, Matthew E.
Advisor(s)
Lam, Wilbur A.
Bryksin, Anton
Bryksin, Anton
Editor(s)
Collections
Supplementary to
Permanent Link
Abstract
Blood clots cause roughly 100,000 deaths each year making it an important health concern. Blood clots form as fibrinogen is polymerized by the enzyme thrombin, forming a
fibrin clot structure. Current anticoagulant and fibrinolytic treatment approaches, including heparin and plasminogen activators, respectively, introduce the risk of bleeding when ad-
ministered systemically due to the inability to differentiate a bad clot (causing heart attack or stroke) from a good clot (preventing bleeding). Engineered bacterial surface display of
fibrinolytic proteins is an innovative synthetic biology approach to degrading fibrin while minimizing the bleeding risk, through enhanced release profiles and antibiotics, yet possess the issue of non-localized treatment. Here, methods to identify a fibrin-specific single stranded DNA (ssDNA) aptamer that exclusively binds to fibrin clots is proposed. Discovery of a fibrin-specific aptamer has many applications, including targeted delivery of engineered E. coli via biotin-streptavidin conjugation and as a biosensor for blood clot detection in laboratory or clinical settings. A fibrin clot, ssDNA library, Gibson assembly primers, dsDNA probes, dsDNA probe-mag bead conjugates, and dsDNA probe-cell conjugates were created as preliminary work. Utilization of these created products and continuation of the proposed methods provides the opportunity to identify a fibrin-specific
aptamer for targeted delivery and biosensing applications.
Sponsor
Date Issued
2022-05-03
Extent
Resource Type
Text
Resource Subtype
Thesis