Title:
Development of a Molecular Monitoring Tool for Imperiled Herpetofauna of the Southeastern U.S.

dc.contributor.author McKee, Anna Miriam en_US
dc.contributor.author Calhoun, Daniel en_US
dc.contributor.author Barichivish, William en_US
dc.contributor.author Spear, Stephens en_US
dc.contributor.author Coldberg, Caren en_US
dc.contributor.author Glenn, Travis en_US
dc.contributor.corporatename USGS Georgia Water Science Center en_US
dc.contributor.corporatename Southeast Ecological Science Center (U.S.) en_US
dc.contributor.corporatename Orianne Society en_US
dc.contributor.corporatename University of Idaho. Dept. of Fish & Wildlife Sciences en_US
dc.contributor.corporatename University of Georgia. Dept. of Environmental Health Science en_US
dc.date.accessioned 2014-03-26T20:15:35Z
dc.date.available 2014-03-26T20:15:35Z
dc.date.issued 2013-04
dc.description Proceedings of the 2013 Georgia Water Resources Conference, April 10-11, 2013, Athens, Georgia. en_US
dc.description.abstract Molecular techniques have recently been demonstrated to be effective and efficient methods for detecting presence of species across a range of aquatic systems. The general method includes the isolation of environmental DNA (eDNA) from focal taxa via water filtration and DNA amplification. The simplicity, costeffectiveness, and non-invasiveness of these techniques for detecting presence of rare and cryptic species suggests great potential for incorporation into inventory and monitoring programs of aquatic species. In this study, we developed and tested eDNA markers for several imperiled pond-breeding amphibian species of Georgia and Florida; the flatwoods salamanders (Ambystoma bishopi and A. cingulatum), gopher frog (Rana capito), and striped newt (Notophthalmus perstriatus), under both lab and field conditions. We report the effectiveness of these markers to detect species presence under both controlled and natural conditions and discuss the potential for these markers to be incorporated into inventory and monitoring programs of amphibians in the southeastern U.S. en_US
dc.description.sponsorship Sponsored by: Georgia Environmental Protection Division; U.S. Department of Agriculture, Natural Resources Conservation Service; Georgia Institute of Technology, Georgia Water Resources Institute; The University of Georgia, Water Resources Faculty. en_US
dc.description.statementofresponsibility This book was published by Warnell School of Forestry and Natural Resources, The University of Georgia, Athens, Georgia 30602-2152. The views and statements advanced in this publication are solely those of the authors and do not represent official views o en_US
dc.identifier.uri http://hdl.handle.net/1853/51483
dc.language.iso en_US en_US
dc.publisher Georgia Institute of Technology en_US
dc.relation.ispartofseries GWRI2013. Ecological: Function, Restoration, Monitoring en_US
dc.subject Water resources management en_US
dc.subject Ecosystem conditions en_US
dc.subject Molecular monitoring en_US
dc.title Development of a Molecular Monitoring Tool for Imperiled Herpetofauna of the Southeastern U.S. en_US
dc.type Text
dc.type.genre Proceedings
dspace.entity.type Publication
local.contributor.corporatename Georgia Water Resources Institute
local.contributor.corporatename School of Civil and Environmental Engineering
local.contributor.corporatename College of Engineering
local.relation.ispartofseries Georgia Water Resources Conference
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relation.isSeriesOfPublication e0bfffc9-c85a-4095-b626-c25ee130a2f3
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