The mechanism of proteoglycan membrane anchorage affects the endocytic pathway of cellular cargo

Hardin, Kevin

Title:

The mechanism of proteoglycan membrane anchorage affects the endocytic pathway of cellular cargo

dc.contributor.author	Hardin, Kevin
dc.contributor.corporatename	Georgia Institute of Technology. Undergraduate Research Opportunities Program
dc.contributor.corporatename	Georgia Institute of Technology. School of Chemistry and Biochemistry
dc.date.accessioned	2009-04-21T16:21:51Z
dc.date.available	2009-04-21T16:21:51Z
dc.date.issued	2009-02-09
dc.description	A presentation in the Undergraduate Research Kaleidoscope: Library East Commons Performance Space, February 9, 2009	en
dc.description	Runtime: 06:43 minutes (Original)
dc.description	Runtime: 06:43 minutes (Edited)
dc.description.abstract	Endocytosis is the process by which the cell is able to take up materials from the extracellular space into the cytosol by enclosing it within its cell membrane. Mechanistically, this is often accomplished by a receptor mediated pathway, which is often controlled by surface molecules known as heparin sulfate proteoglycans (HSPGs). Study of the control of these pathways is therefore of keen interest for the pharmaceutical industry because endocytosis may be used for drug and gene delivery vectors as well as to prevent viruses and other toxins from entering the cell. These proteoglycans may be bound to the cell membrane using a transmembrane core protein such as syndecans or through the use of glycosylphosphatidylinositol (GPI) anchors such as glypicans. It has been proposed that the endocytic pathway followed will vary depending on whether the proteoglycan is membrane bound via a GPI-anchor or using a transmembrane core protein. The HSPGs have a typical half-life on the surface of the cell membrane ranging from 3-8 hours and afterwards the molecule is either endocytosed or is shed into the extracellular space. If the HSPG is bound with a GPI-anchor the endocytosis will lead immediately to the lysosome for degradation. This pathway is in stark contrast to the HSPGs employing a transmembrane core protein, which undergoes several degradations within prelysosomal compartments steps prior to its final digestion within the lysosome. Immediately after endocytosis the core protein is broken down to its constituent HS chains which are cleaved by endoglycosidases followed by further digestion by endoglycosidases which occurs in a distinct compartment from the previous step where it is finally transferred to the lysosome. The aim of this research will involve observation of the endocytosis of GPI-linked HSPGs to confirm the proposed mechanisms and furthermore to remove the GPI linkage using phospholipases to determine if it is possible to direct cargo into the pathway followed by transmembrane linked HSPGs.	en
dc.description.advisor	Christine Payne, Payne Lab
dc.format.extent	06:43 minutes (Original)
dc.format.extent	06:43 minutes (Edited)
dc.identifier.uri	http://hdl.handle.net/1853/27728
dc.language.iso	en_US	en
dc.publisher	Georgia Institute of Technology	en
dc.relation.ispartofseries	Undergraduate Research Symposia
dc.subject	Proteoglycans	en
dc.subject	Endocytic pathway	en
dc.title	The mechanism of proteoglycan membrane anchorage affects the endocytic pathway of cellular cargo	en
dc.type	Moving Image
dc.type	Text
dc.type.genre	Presentation
dspace.entity.type	Publication
local.contributor.corporatename	Undergraduate Research Opportunities Program
local.relation.ispartofseries	Undergraduate Research Kaleidoscope
relation.isOrgUnitOfPublication	0db885f5-939b-4de1-807b-f2ec73714200
relation.isSeriesOfPublication	85f7406f-e4f0-4862-857f-15cde3608d3e