Title:
Screening for the Functionality of RNA Templated Repair of Double-Strand Breaks in Saccharomyces Cerevisae

dc.contributor.advisor Storici, Francesca
dc.contributor.author Gordon, Katherine
dc.contributor.committeeMember Meers, Chance
dc.contributor.department Biology
dc.date.accessioned 2017-07-28T18:32:52Z
dc.date.available 2017-07-28T18:32:52Z
dc.date.created 2017-05
dc.date.issued 2017-05
dc.date.submitted May 2017
dc.date.updated 2017-07-28T18:32:52Z
dc.description.abstract Double-strand breaks (DSBs) in DNA are detrimental, as they can cause mutations and genomic rearrangements, which in turn leads to cancer and other diseases. Recent research reveals that DSBs can be repaired by RNA-templated homologous recombination. However, RNA-templated repair of DSBs is not well understood. In order to better understand the mechanism of RNA-templated repair of DSBs, the current research aims to identify the proteins that facilitate the repair. The research utilizes a system wherein RNA-templated repair of DSBs is known to occur. A yeast overexpression plasmid library was produced in order to test the ability of fragments of the yeast genome to facilitate RNA-templated repair of a DSB when these are highly expressed in the yeast cells. In order to test the ability of added gene fragments to facilitate RNA-templated repair of DSBs, the experimental candidates were exposed to galactose in order to induce a DSB, to activate the transcription of the overexpressed gene fragment, and to initiate the transcription of anti-sense RNA used to repair the break. The candidates were then moved to medium without histidine in order to assess the frequency of repair. Once a large number of colonies (~50,000) are screened, we expect to identify several proteins that facilitate RNA-templated repair of DSBs. Identifying the specific genes that facilitate this repair mechanism will assist in characterizing the functionality of the RNA-templated DNA repair mechanism. Identifying these genes will also allow for better predictions for how this same phenomenon may occur in human cells.
dc.description.degree Undergraduate
dc.format.mimetype application/pdf
dc.identifier.uri http://hdl.handle.net/1853/58472
dc.publisher Georgia Institute of Technology
dc.subject DNA Repair
dc.subject Double-Strand Breaks
dc.subject RNA-templated repair
dc.title Screening for the Functionality of RNA Templated Repair of Double-Strand Breaks in Saccharomyces Cerevisae
dc.type Text
dc.type.genre Undergraduate Thesis
dspace.entity.type Publication
local.contributor.advisor Storici, Francesca
local.contributor.corporatename College of Sciences
local.contributor.corporatename School of Biological Sciences
local.contributor.corporatename Undergraduate Research Opportunities Program
local.relation.ispartofseries Undergraduate Research Option Theses
relation.isAdvisorOfPublication 94282868-8eb6-4f1b-84b5-db8df7ae95a9
relation.isOrgUnitOfPublication 85042be6-2d68-4e07-b384-e1f908fae48a
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thesis.degree.level Undergraduate
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