Title:
The effect of biochemical stimulation on mechanical properties of collagen-based tissue engineered blood vessels

dc.contributor.author Jackman, Chris en_US
dc.contributor.department Biomedical Engineering en_US
dc.date.accessioned 2011-05-11T19:15:24Z
dc.date.available 2011-05-11T19:15:24Z
dc.date.issued 2011-05-09 en_US
dc.description.abstract The successful creation of a living blood vessel substitute for bypass surgery can overcome current problems associated with grafting the patient’s own vascular tissue. A fully biological vessel comprised of smooth muscle cells (SMC's) in collagen is an attractive alternative due to its potential for long-term remodeling and vasoactivity, but these vessels are limited by inherent physical weaknesses. This study determines how the mechanical properties of tissue engineered blood vessels made with the collagen/SMC method can be improved by biochemical stimulation. Vessels were prepared by embedding rat aortic smooth muscle cells in a collagen gel and culturing the constructs for two weeks to allow matrix remodeling. Experimental vessels were treated with transglutaminase and cultured in media supplemented with ascorbic acid, TGF-beta, and insulin. Mechanical properties of these vessels were measured by using tensile testing of ring samples from the vessels and applying internal pressure loads over whole constructs. The treated vessels demonstrated statistically significant improvements in both ultimate tensile strength and burst pressure relative to control vessels. These results indicate that biochemical stimulation can facilitate improvements in mechanical properties of tissue engineered blood vessels. en_US
dc.description.advisor Gleason, Rudy - Faculty Mentor ; Zamir, Evan - Committee Member/Second Reader en_US
dc.identifier.uri http://hdl.handle.net/1853/38829
dc.language.iso en_US en_US
dc.publisher Georgia Institute of Technology en_US
dc.subject Cardiovascular system en_US
dc.subject Smooth muscle cells en_US
dc.subject Bypass surgery en_US
dc.title The effect of biochemical stimulation on mechanical properties of collagen-based tissue engineered blood vessels en_US
dc.type Text
dc.type.genre Undergraduate Thesis
dspace.entity.type Publication
local.contributor.corporatename Wallace H. Coulter Department of Biomedical Engineering
local.contributor.corporatename Undergraduate Research Opportunities Program
local.contributor.corporatename College of Engineering
local.relation.ispartofseries Undergraduate Research Option Theses
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relation.isOrgUnitOfPublication 7c022d60-21d5-497c-b552-95e489a06569
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