Title:
The role of CheC and McpB in the chemotaxis system of Bacillus subtilis
The role of CheC and McpB in the chemotaxis system of Bacillus subtilis
Author(s)
Paglioni, Sarah
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Abstract
Chemotaxis is one of many two-component signal transduction (TCST) systems found in
cells. B. subtilis shares a very similar chemotaxis system to many other bacteria and
archaea and thus studies of B. subtilis can be extrapolated to the systems in other
organisms. To further elucidate the current model of chemotaxis in B. subtilis we used
the yeast-two-hybrid and β-galactosidase assays to determine the interactions between
CheC and CheA and also between McpB and CheY. Our results reveal the interaction of
CheC with the HPT domain of the histidine kinase, CheA. Interestingly, the introduction
of a mutation near the phosphatase region of CheC completely disrupts the interaction
with CheA. The interaction between CheA and CheC may serve as yet another level of
regulation to the system. Furthermore, we explore the possibility of the receptor itself
interacting with the response regulator to also aid in the adaptation of the signal.
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Date Issued
2007-05
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Text
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Undergraduate Thesis